High-frequency intracellular invasion of epithelial cells by serotype M1 group A streptococci: M1 protein-mediated invasion and cytoskeletal rearrangements

被引:104
作者
Dombek, PE
Cue, D
Sedgewick, J
Lam, H
Ruschkowski, S
Finlay, BB
Cleary, PP
机构
[1] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Cell Biol & Neuroanat, Minneapolis, MN 55455 USA
[3] Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1W5, Canada
[4] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1W5, Canada
[5] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1W5, Canada
关键词
D O I
10.1046/j.1365-2958.1999.01223.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A clonal variant of serotype M1 group A streptococcus (designated M1inv(+)) has been linked to severe and invasive infections, including sepsis, necrotizing fasciitis and toxic shock: High frequency internalization of cultured epithelial cells by the M1inv(+) strain 90-226 is dependent upon the M1 protein. Invasion of HeLa cells was blocked by an anti-M1 antibody, invasion by an M1(-) strain (90-226 emm1::km) was greatly reduced, and latex beads bound to M1 protein were readily internalized by HeLa cells. Beads coated with a truncated M1 protein were internalized far less frequently. Scanning electron microscopy indicated that streptococci invade by a zipper-like mechanism, that may be mediated by interactions with host cell microvilli. Initially, internalized streptococci and streptococci undergoing endocytosis are associated with polymerized actin. Later in the internalization process, streptococcal-containing vacuoles are associated with the lysosomal membrane glycoprotein, LAMP-1.
引用
收藏
页码:859 / 870
页数:12
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