State of human papilloma virus DNA in cervical carcinomas

被引：7
作者：
Kisseljov, FL [1 ]
Kisseljova, NP
Kobzeva, VK
Gritsko, TM
Semenova, LA
Pavlova, LS
Klaes, R
Doeberitz, MV
机构：
[1] Russian Acad Med Sci, Blokhin Canc Res Ctr, Moscow 115478, Russia
[2] Univ Heidelberg, D-69120 Heidelberg, Germany
来源：
MOLECULAR BIOLOGY | 2001年 / 35卷 / 03期
基金：
俄罗斯基础研究基金会;
关键词：
papilloma virus; cervical carcinoma; DNA; integration; episome; expression;
D O I：
10.1023/A:1010430913634
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cervical carcinoma is etiologically associated with the human papilloma virus (HPV), HPV 16 and HPV 18 being the most common. Viral DNA is thought to persist mostly in the episomal form in early tumor development, and in the integrated form in carcinomas. This assumption was checked with a new method that discriminated between RNAs transcribed from episomal and integrated HPV DNAs. Both forms were detected in carcinomas of Russian patients regardless of the disease stage. The data were verified by two other methods. RNA with sequences of the HPV transforming gene E7 proved to be transcribed from either DNA form. The results suggest that HPV integration is not crucial for carcinoma progression.
引用
收藏
页码：399 / 404
页数：6
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