Human-engineered auricular reconstruction (hEAR) by 3D-printed molding with human-derived auricular and costal chondrocytes and adipose-derived mesenchymal stem cells

被引：18

作者：

Landau, Shira ^{[1
]}

Szklanny, Ariel A. ^{[1
]}

Machour, Majd ^{[1
]}

Kaplan, Ben ^{[1
]}

Shandalov, Yulia ^{[1
]}

Redenski, Idan ^{[1
]}

Beckerman, Margarita ^{[1
]}

Harari-Steinberg, Orit ^{[2
]}

Zavin, Janet ^{[1
]}

Karni-Katovitch, Oryan ^{[1
]}

Goldfracht, Idit ^{[1
]}

Michael, Inbal ^{[1
]}

Waldman, Stephen D. ^{[3
]}

Duvdevani, Shay, I ^{[2
,4
]}

Levenberg, Shulamit ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Fac Biomed Engn, Haifa, Israel

[2] Sheba Med Ctr, Tissue Engn Lab, Ramat Gan, Israel

[3] Ryerson Univ, Dept Chem Engn, Toronto, ON, Canada

[4] Sheba Med Ctr, Dept Otorhinolaryngol Head & Neck Surg, IL-5262166 Ramat Gan, Israel

来源：

BIOFABRICATION | 2022年 / 14卷 / 01期

基金：

欧洲研究理事会;

关键词：

auricle; chondrogenesis; engineered tissue; microtia; EAR-SHAPED CARTILAGE; MICROTIA CHONDROCYTES; TISSUE; REGENERATION;

D O I：

10.1088/1758-5090/ac3b91

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Microtia is a small, malformed external ear, which occurs at an incidence of 1-10 per 10 000 births. Autologous reconstruction using costal cartilage is the most widely accepted surgical microtia repair technique. Yet, the method involves donor-site pain and discomfort and relies on the artistic skill of the surgeon to create an aesthetic ear. This study employed novel tissue engineering techniques to overcome these limitations by developing a clinical-grade, 3D-printed biodegradable auricle scaffold that formed stable, custom-made neocartilage implants. The unique scaffold design combined strategically reinforced areas to maintain the complex topography of the outer ear and micropores to allow cell adhesion for the effective production of stable cartilage. The auricle construct was computed tomography (CT) scan-based composed of a 3D-printed clinical-grade polycaprolactone scaffold loaded with patient-derived chondrocytes produced from either auricular cartilage or costal cartilage biopsies combined with adipose-derived mesenchymal stem cells. Cartilage formation was measured within the construct in vitro, and cartilage maturation and stabilization were observed 12 weeks after its subcutaneous implantation into a murine model. The proposed technology is simple and effective and is expected to improve aesthetic outcomes and reduce patient discomfort.

引用

页数：14