Fetoscopic coverage of experimental myelomeningocele in sheep using a patch with surgical sealant

Objective: Assess the feasibility of a fetoscopic patch coverage method for myelomeningocele repair in a sheep model. Study design: Experimental study. A myelomeningocele-like defect was created in 15 fetal sheep on day 75 of gestation. Six remained untreated, whereas 9 underwent fetoscopic coverage of the defect on day 95 of gestation using an inert patch secured with surgical sealant. Clinical and histological examinations were performed after delivery. Results: Four valid newborn lambs were obtained in each group. Mean fetoscopic surgical time was 26.9 (SD = 7.4) min. All untreated animals had an open lumbar defect with cerebrospinal fluid leakage, paraplegia, urinary incontinence, and Chiari malformation. All treated animals had a closed defect and were able to walk: one had weak bladder control, and another mild Chiari malformation. Conclusion: In a chronic myelomeningocele model in fetal sheep, fetoscopic repair using a sealed patch results in simple, fast, satisfactory neural tube closure and averts neurological damage and Chiari malformation. (C) 2011 Elsevier Ireland Ltd. All rights reserved.