Recombinase amplified CRISPR enhanced chain reaction (RACECAR) for viral genome detection

被引：5
作者：
Kachwala, Mahera J. [1 ]
Smith, Christopher W. [1 ]
Nandu, Nidhi [1 ]
Yigit, Mehmet, V [1 ,2 ]
机构：
[1] SUNY Albany, Dept Chem, 1400 Washington Ave, Albany, NY 12222 USA
[2] SUNY Albany, RNA Inst, 1400 Washington Ave, Albany, NY 12222 USA
来源：
NANOSCALE | 2022年 / 14卷 / 37期
基金：
美国食品与农业研究所;
关键词：
POLYMERASE AMPLIFICATION; DNA; CRISPR-CAS12A; DIAGNOSTICS; GENOTYPE; PLATFORM; ASSAY;
D O I：
10.1039/d2nr03590a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed a 'recombinase amplified CRISPR enhanced chain reaction' (RACECAR) assay that can detect as little as 40 copies of hepatitis B virus (HBV) genome using a benchtop spectrofluorometer. The limit of detection was determined to be 3 copies of HBV genome. The specificity of RACECAR was confirmed against hepatitis A virus (HAV). This assay can detect the genomic targets directly in serum samples without an extraction step. The 4 h-long fluorometric assay was developed by combining three tiers of isothermal amplification processes and can be repurposed for any target of choice. This highly modular reaction setup is an untapped resource that can be incorporated into the front-runners of molecular diagnostics.
引用
收藏
页码：13500 / 13504
页数：5
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