Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

被引：11

作者：

Balashev, Konstantin ^{[1
,2
]}

Callisen, Thomas H. ^{[3
]}

Svendsen, Allan ^{[3
]}

Bjornholm, Thomas ^{[2
]}

机构：

[1] Univ Sofia, Dept Phys Chem, Biophys Chem Lab, Sofia 1164, Bulgaria

[2] Univ Copenhagen, Dept Chem, Nanosci Ctr, DK-2100 Copenhagen O, Denmark

[3] Novozymes, DK-2880 Bagsvaerd, Denmark

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 88卷 / 02期

关键词：

Bovine serum albumin (BSA) monolayers; Savinase; Enzyme hydrolysis; Atomic Force Microscopy (AFM); PHOSPHOLIPASE A(2) HYDROLYSIS; HUMICOLA-LANUGINOSA LIPASE; ENZYMATIC-HYDROLYSIS; KINETICS; SPREAD;

D O I：

10.1016/j.colsurfb.2011.07.043

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：582 / 586

页数：5

共 24 条

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