Disposable electrochemical DNA-array for PCR amplified detection of hazelnut allergens in foodstuffs

被引：66

作者：

Bettazzi, Francesca ^{[1
,2
]}

Lucarelli, Fausto ^{[1
]}

Palchetti, Ilaria ^{[1
]}

Berti, Francesca ^{[1
]}

Marrazza, Giovanna ^{[1
]}

Mascini, Marco ^{[1
,2
]}

机构：

[1] Univ Florence, Dipartmento Chim, I-50019 Sesto Fiorentino, FI, Italy

[2] INBB, I-00136 Rome, Italy

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 614卷 / 01期

关键词：

electrochemical genosensor; food allergens; hazelnut; polymerase chain reaction; screen-printed electrode; array;

D O I：

10.1016/j.aca.2008.03.027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical low-density DNA-array has been designed and implemented to be used in combination with polymerase chain reaction (PCR) in order to investigate the presence of hazelnut major allergens (Cor a 1.04, Cor a 1.03) in foodstuff. Unmodified PCR products were captured at the sensor interface via sandwich hybridization with surface-tethered probes and biotinylated signalling probes. The resulting biotinylated hybrids were coupled with a streptavidin-alkaline phosphatase conjugate and then exposed to a alpha-naphthyl phosphate solution. Differential pulse voltammetry was finally used to detect the alpha-naphthol signal. The detection limits for Cor a 1.03 and Cor a 1.04 were 0.3 and 0.1 nmol L-1, respectively (R.S.D. 10%). The optimized conditions were used to test several commercially available foodstuffs, claiming to contain or not the targeted nuts. The results were compared with those obtained with classical ELISA tests. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：93 / 102

页数：10

共 24 条

[1] Detection of hazelnut (Corylus spp.) in processed foods using real-time PCR [J].

Arlorio, M. ;

Cereti, E. ;

Coisson, J. D. ;

Travaglia, F. ;

Martelli, A. .

FOOD CONTROL, 2007, 18 (02) :140-148

[2] Immunological identification and characterization of individual food allergens [J].

Becker, WM ;

Reese, G .

JOURNAL OF CHROMATOGRAPHY B, 2001, 756 (1-2) :131-140

[3] Determination of allergens in foods [J].

Besler, M .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2001, 20 (11) :662-672

[4] ENHANCED DNA-SEQUENCING BY HYBRIDIZATION [J].

BROUDE, NE ;

SANO, T ;

SMITH, CL ;

CANTOR, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3072-3076

[5]

*COD AL COM FAO WH, 1998, 26 SESS COD COMM FOO

[6] Steric factors controlling the surface hybridization of PCR amplified sequences [J].

Del Giallo, ML ;

Lucarelli, F ;

Cosulich, E ;

Pistarino, E ;

Santamaria, B ;

Marrazza, G ;

Mascini, M .

ANALYTICAL CHEMISTRY, 2005, 77 (19) :6324-6330

[7] Disposable electrochemical genosensor for the simultaneous analysis of different bacterial food contaminants [J].

Farabullini, F. ;

Lucarelli, F. ;

Palchetti, I. ;

Marrazza, G. ;

Mascini, M. .

BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) :1544-1549

[8] A DNA-SEQUENCING STRATEGY THAT REQUIRES ONLY 5 BASES OF KNOWN TERMINAL SEQUENCE FOR PRIMING [J].

FU, DJ ;

BROUDE, NE ;

KOSTER, H ;

SMITH, CL ;

CANTOR, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) :10162-10166

[9] Polymerase chain reaction coupled with peptide nucleic acid high-performance liquid chromatography for the sensitive detection of traces of potentially allergenic hazelnut in foodstuffs [J].

Germini, A ;

Scaravelli, E ;

Lesignoli, F ;

Sforza, S ;

Corradini, R ;

Marchelli, R .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2005, 220 (5-6) :619-624

[10] Detection of peanut using real-time polymerase chain reaction [J].

Hird, H ;

Lloyd, J ;

Goodier, R ;

Brown, J ;

Reece, P .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2003, 217 (03) :265-268

← 1 2 3 →