Hexavalent Chromium-Induced Apoptosis in Rat Uterus: Involvement of Oxidative Stress

被引：12

作者：

Marouani, Neila ^{[1
]}

Tebourbi, Olfa ^{[1
]}

Mokni, Moncef ^{[2
]}

Yacoubi, Mohamed Tahar ^{[2
]}

Sakly, Mohsen ^{[1
]}

Benkhalifa, Moncef ^{[3
,4
]}

Ben Rhouma, Khemais ^{[1
]}

机构：

[1] Fac Sci Bizerte, Lab Physiol Integree, Zarzouna, Tunisia

[2] Hop Univ Farhat Hached, Serv Anat & Cytol Pathol, Sousse, Tunisia

[3] ATL R&D, Reprod Biol & Genet Lab, Paris La Verriere, France

[4] Unilabs Grp, Geneva, Switzerland

来源：

ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH | 2015年 / 70卷 / 04期

关键词：

apoptosis; hexavalent chromium; oxidative stress; rat; uterus; DEFENSE-MECHANISMS; SEXUAL-MATURATION; DNA-DAMAGE; CYTOTOXICITY; MITOCHONDRIA; SUPEROXIDE; EXPOSURE; CR(VI);

D O I：

10.1080/19338244.2013.828673

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The present study is designed to test the hypothesis that oxidative stress mediates hexavalent chromium (VI)-induced apoptosis in uterus. Female Wistar rats received an intraperitoneal (i.p.) injection of potassium dichromate at doses of 1 and 2mg/kg. Superoxide anion production was assessed by determination of the reduction of cytochrome c and iodonitrotetrazolium (INT), lipid peroxidation (LPO), metallothioneins (MTs), and catalase (CAT) activity. The expression of Bax and Bcl-2 proteins was investigated. After 15days of treatment, an increase of LPO and MT levels occurred, whereas CAT activity decreased. Intense apoptosis was observed in endometriotic stromal cells of Cr-exposed rats. Bax protein expression was induced in endometriotic stromal cells with 1mg of Cr(VI)/kg, and in stromal and epithelial cells at the higher dose. These results clearly suggest that Cr(VI) subacute treatment causes oxidative stress in rat uterus, leading to endometriotic stromal cells apoptosis.

引用

页码：189 / 195

页数：7

共 38 条

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