Epitope peptides of influenza H3N2 virus neuraminidase gene designed by immunoinformatics

被引:7
作者
Liang, Lijun [1 ,3 ]
Huang, Ping [1 ,3 ]
Wen, Miaoheng [2 ]
Ni, Hanzhong [1 ]
Tan, Songnuan [2 ]
Zhang, Yonghui [1 ]
Chen, Qiuxia [1 ]
机构
[1] Guangdong Prov Ctr Dis Control & Prevent, Key Lab Emergency Pathogen Detect, Guangzhou 510300, Guangdong, Peoples R China
[2] Sinoasis Pharmaceut Guangzhou Inc, Guangzhou 510760, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Sch Publ Hlth, Guangzhou 510080, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
B-cell epitope; H3N2; immunoinformatics; neuraminidase (NA); A VIRUS; H1N1;
D O I
10.1093/abbs/gmr101
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The virus surface protein neuraminidase (NA) is a main subtype-specific antigen in influenza type A viruses. Neuraminidase functions as an enzyme to break the bonds between hemagglutinin (HA) and sialic acid to release newly formed viruses from infected cells. In this study, NA genes from the H3N2 subtype virus were sequenced and NA proteins were screened for B-cell epitopes and assessed based on immunoinformatics. Based on this information, three peptides ES8, RR9, and WK7 (covering amino acid residues 221-228, 292-300, and 383-389, respectively) of the NA protein were selected and synthesized artificially. These peptides were used to immunize New Zealand rabbits subcutaneously to raise antisera. Results showed that these three peptides were capable of eliciting antibodies against H3N2 viruses in a specific and sensitive manner, detected in vitro by enzyme-linked immunosorbent assay. Furthermore, hemadsorption anti-releasing effects occurred in three antisera mixtures at a dilution of 1:40. Alignment using database software showed that amino acid residues in these three epitope peptides were substituted at specific sites in all the NAs sequenced in this study. We suggest that these NA epitope peptides might be used in conjunction with HA proteins as vaccine antigens.
引用
收藏
页码:113 / 118
页数:6
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