Rapid modification of bacterial artificial chromosomes by ET-recombination

被引：407

作者：

Muyrers, JPP ^{[1
]}

Zhang, YM ^{[1
]}

Testa, G ^{[1
]}

Stewart, AF ^{[1
]}

机构：

[1] European Mol Biol Lab, Gene Express Program, D-69117 Heidelberg, Germany

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 06期

关键词：

D O I：

10.1093/nar/27.6.1555

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

引用

页码：1555 / 1557

页数：3

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