Dissection and validation of a QTL cluster linked toRht-B1locus controlling grain weight in common wheat (Triticum aestivumL.) using near-isogenic lines

被引：36

作者：

Guan, Panfeng ^{[1
,2
]}

Shen, Xueyi ^{[1
]}

Mu, Qing ^{[1
]}

Wang, Yongfa ^{[1
]}

Wang, Xiaobo ^{[1
]}

Chen, Yongming ^{[1
]}

Zhao, Yue ^{[1
]}

Chen, Xiyong ^{[3
]}

Zhao, Aiju ^{[3
]}

Mao, Weiwei ^{[1
]}

Guo, Yiwen ^{[1
]}

Xin, Mingming ^{[1
]}

Hu, Zhaorong ^{[1
]}

Yao, Yingyin ^{[1
]}

Ni, Zhongfu ^{[1
]}

Sun, Qixin ^{[1
]}

Peng, Huiru ^{[1
]}

机构：

[1] China Agr Univ, Key Lab Crop Heterosis & Utilizat, Beijing Key Lab Crop Genet Improvement,Minist Edu, Coll Agron & Biotechnol,State Key Lab Agrobiotech, Beijing 100193, Peoples R China

[2] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China

[3] Hebei Acad Agr & Forestry Sci, Hebei Crop Genet Breeding Lab, Inst Cereal & Oil Crops, Shijiazhuang 050035, Hebei, Peoples R China

来源：

THEORETICAL AND APPLIED GENETICS | 2020年 / 133卷 / 09期

基金：

中国博士后科学基金;

关键词：

YIELD-RELATED TRAITS; GENOME-WIDE ASSOCIATION; GENETIC DISSECTION; DWARFING GENES; BREAD WHEAT; SIZE; LOCI; DOMESTICATION; ENVIRONMENTS; IMPROVEMENT;

D O I：

10.1007/s00122-020-03622-z

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Key message This study dissected and validated a QTL cluster associated with thousand grain weight on chromosome 4B using multiple near-isogenic lines in common wheat. Grain size and weight are crucial components of wheat yield. Previously, we identified a QTL cluster for thousand grain weight (TGW) on chromosome 4B using the Nongda3338 (ND3338)/Jingdong6 (JD6) doubled haploid population. Here, near-isogenic lines (NILs) in the ND3338 background were developed to dissect and validate the QTL cluster. Based on six independent BC(3)F(3:4)heterogeneous inbred families, the 4B QTL cluster was divided into two linked QTL intervals (designated 4B.1 and 4B.2 QTL). For the 4B.1 QTL, theRht-B1gene, of whichRht-B1ballele reduces plant height (PH) by 21.18-29.34 cm (34.34-53.71%), was demonstrated to be the most likely candidate gene with pleiotropic effects on grain size and TGW. For the 4B.2 QTL, the NIL(JD6)consistently showed an increase in TGW of 3.51-7.68 g (8.84-22.77%) compared with NIL(ND3338)across different field trials, along with a significant increase in PH of 2.26-6.71 cm (3.92-12.01%). Moreover, both QTL intervals had a larger effect on grain width than on grain length. Additionally, the first significant difference in 100-grain fresh weight and 100-grain dry weight between the NIL pairs of the 4B.1 QTL interval (Rht-B1) was observed at 6 days after pollination (DAP), while the differences were first visible at 30 DAP for the 4B.2 QTL interval. Collectively, our work provides a new example of QTL dissection for grain weight in wheat and lays a foundation for further map-based cloning of the major QTL that have potential applications in wheat molecular breeding for high yield.

引用

页码：2639 / 2653

页数：15

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