Multiplex PCR for the simultaneous detection of Salmonella spp. and Salmonella Enteritidis in food

被引：32
|
作者：
Pinto Silva, Daniel Santos [1 ]
Canato, Thayse [1 ]
Magnani, Marciane [1 ]
Alves, Juliane [1 ]
Hirooka, Elisa Yoko [1 ]
Rocha Moreira de Oliveira, Tereza Cristina [1 ]
机构：
[1] Univ Estadual Londrina, Ctr Ciencias Agr, Dept Ciencia & Tecnol Alimentos, BR-87051970 Londrina, PR, Brazil
来源：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2011年 / 46卷 / 07期
关键词：
Chicken carcasses; multiplex PCR; pork sausages; Salmonella Enteritidis; Salmonella spp; POLYMERASE-CHAIN-REACTION; MELTING-PEAK ANALYSIS; ESCHERICHIA-COLI; LISTERIA-MONOCYTOGENES; CAMPYLOBACTER-JEJUNI; ASSAY; TYPHIMURIUM; CHICKEN; PREENRICHMENT; VALIDATION;
D O I：
10.1111/j.1365-2621.2011.02646.x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Multiplex PCR assay (mPCR) for the detection of Salmonella spp. and S. Enteritidis was developed in this study using artificially contaminated chicken carcasses. The assay showed 100% specificity to detect approximately 1 CFU of Salmonella in 10 g of chicken skin after non-selective enrichment. The mPCR was evaluated in Minas cheese, fresh pork sausage and chicken carcasses commercially available. Salmonella spp. was detected in nine of sixty-six chicken carcasses, five of fifty-two cheese samples, and five of fifty-two sausage samples. The serovar Enteritidis was detected in two samples of contaminated sausage. The mPCR results were confirmed by conventional culture and biochemical identification of the isolates. Serotyping confirmed the presence of S. Enteritidis in sausage samples and showed contamination by serovars Schwarzengrund and Montevideo in chicken carcasses.
引用
收藏
页码：1502 / 1507
页数：6
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