Expression profile of plasma microRNAs in nonsyndromic cleft lip and their clinical significance as biomarkers

被引：19
作者：
Zou, Jijun [1 ]
Li, Jingyun [2 ]
Li, Jun [2 ]
Ji, Chenbo [3 ]
Li, Qingping [4 ]
Guo, Xirong [3 ]
机构：
[1] Nanjing Med Univ, Nanjing Childrens Hosp, Dept Burns & Plast Surg, Gulou Area, 72 Guangzhou Rd, Nanjing 210008, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Dept Plast & Cosmet Surg, State Key Lab Reprod Med, 123rd Tianfei St,Mochou Rd, Nanjing 210004, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Dept Children Hlth Care, 123rd Tianfei St,Mochou Rd, Nanjing 210004, Jiangsu, Peoples R China
[4] Nanjing Med Univ, Collaborat Innovat Ctr Cardiovasc Dis Translat Me, Dept Pharmacol, Nanjing 210029, Jiangsu, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2016年 / 82卷
基金：
中国国家自然科学基金;
关键词：
Nonsyndromic cleft lip; Plasma microRNA; Biomarker; miRNA microarray; MIRNA SIGNATURES; BINDING-SITE; PALATE; FLUID;
D O I：
10.1016/j.biopha.2016.05.033
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Objective: The aim of this study was to determine the expression profile of plasma microRNAs in nonsyndromic cleft lip (NSCL) and their clinical significance as biomarkers. Methods: Agilent human miRNA microarray chips were used to analyze three NSCL plasma samples (mixed as CL group) and three normal plasma samples (mixed as Control group). Six selected plasma miRNAs were validated using qRT-PCR between another 13 CL and 11 healthy children. The receiver operating characteristic (ROC) curve analysis was applied for three elevated miRNAs, miR-16-2-3p, miR-365a-3p and miR-877-5p. Their target genes were further assessed using gene ontology and pathway analysis. Results: The plasma miRNA differentially expressed (fold change >= 2) amounted to 305. In particular, it had been validated that miR-16-2-3p, miR-365a-3p and miR-877-5p were elevated in NSCL plasma samples. ROC curve analysis revealed that each microRNA was able to significantly discriminate NSCL subjects from normal controls. Gene ontology and pathway analysis revealed that many processes over-represented in CL are related to system development process, regulation of nitrogen compound metabolic process, FoxO signaling pathway and the ErbB signaling pathway. Conclusion: Our study demonstrated that plasma miR-16-2-3p, miR-365a-3p and miR-877-5p might become biomarkers to diagnose NSCL and dysregulation of these miRNAs might be involved in the progression of NSCL. (C) 2016 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：459 / 466
页数：8
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