Effect of G-rich sequences on the synthesis, purification, hybridization, cell uptake, and hemolytic activity of oligonucleotides

被引：28

作者：

Agrawal, S

Iadarola, PL

Temsamani, J

Zhao, QY

Shaw, DR

机构：

[1] UNIV ALABAMA,DEPT MED,DIV HEMATOL & ONCOL,BIRMINGHAM,AL 35294

[2] UNIV ALABAMA,DEPT PHARMACOL & TOXICOL,BIRMINGHAM,AL 35294

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 18期

关键词：

D O I：

10.1016/0960-894X(96)00403-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We designed G-rich oligonucleotides in which the position and length of the guanosine residues were modified and studied the effects of these contiguous guanosine residues on the synthesis, purification, hybridization, cell uptake, and hemolytic effect of oligonucleotides. Our results revealed that the hyperstructure formation of these oligonucleotides depended on the length and position of the guanosine residues and the flanking sequences. The phosphorothioate oligonucleotides formed much less hyperstructures than their phosphodiester counterpart. These hyperstructures were more stable towards nucleases, were taken up by cells efficiently, and showed pronounced polyanionic related side-effects. Copyright (C) 1996 Elsevier Science Ltd

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页码：2219 / 2224

页数：6

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