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MicroRNA expression in zebrafish embryonic development
被引:1264
作者:
Wienholds, E
Kloosterman, WP
Miska, E
Alvarez-Saavedra, E
Berezikov, E
de Bruijn, E
Horvitz, HR
Kauppinen, S
Plasterk, RHA
机构:
[1] Netherlands Inst Dev Biol, Hubrecht Lab, Ctr Biomed Genet, NL-3584 CT Utrecht, Netherlands
[2] MIT, Howard Hughes Med Inst, Dept Biol, Cambridge, MA 02139 USA
[3] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
[4] Univ Cambridge, Canc Res UK, Gurdon Inst, Wellcome Trust, Cambridge CB2 1QN, England
[5] Univ Copenhagen, Inst Med Biochem & Genet, Wilhelm Johannsen Ctr Funct Genome Res, DK-2200 Copenhagen, Denmark
来源:
关键词:
D O I:
10.1126/science.1114519
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid-modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.
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页码:310 / 311
页数:2
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