Strong substrate-stabilizing effect of a water-miscible ionic liquid [BMIM][BF4] in the catalysis of horseradish peroxidase

被引：13

作者：

Hong, Eun Sik ^{[1
]}

Kwon, O. Yul ^{[2
]}

Ryu, Keungarp ^{[1
]}

机构：

[1] Univ Ulsan, Coll Engn, Sch Chem Engn & Bioengn, Ulsan 680749, South Korea

[2] Seoul Natl Univ Technol, Dept Environm Engn, Seoul 139743, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 03期

关键词：

horseradish peroxidase; ionic liquid; noncompetitive inhibition; thermodynamics;

D O I：

10.1007/s10529-007-9570-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (2-methoxyphenol) by H2O2 were investigated. The ionic liquid stabilized the ground state of guaiacol by causing an 8-fold increase of K-m from 3 to 23 mM upon the addition of 25% (v/v) [BMIM][BF4]. In addition, the effect of [BMIM][BF4] in decreasing the k(cat) value of HRP catalysis was described by a non-competitive inhibition mechanism. The value of the inhibition constant of [BMIM][BF4] was 2.9 M indicating that the ionic liquid plays the role of a weak non-competitive inhibitor for HRP catalysis.

引用

页码：529 / 533

页数：5

共 11 条

[1] ENZYMATIC REMOVAL OF SELECTED AROMATIC CONTAMINANTS FROM WASTE-WATER BY A FUNGAL PEROXIDASE FROM COPRINUS-MACRORHIZUS IN BATCH REACTORS [J].

ALKASSIM, L ;

TAYLOR, KE ;

NICELL, JA ;

BEWTRA, JK ;

BISWAS, N .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1994, 61 (02) :179-182

[2] Growth of Escherichia coli, Pichia pastoris and Bacillus cereus in the presence of the ionic liquids [BMIM][BF4] and [BMIM][PF6] and organic solvents [J].

Ganske, F ;

Bornscheuer, UT .

BIOTECHNOLOGY LETTERS, 2006, 28 (07) :465-469

[3] Oxidative enzymes possess catalytic activity in systems with ionic liquids [J].

Hinckley, G ;

Mozhaev, VV ;

Budde, C ;

Khmelnitsky, YL .

BIOTECHNOLOGY LETTERS, 2002, 24 (24) :2083-2087

[4] Enzyme catalysis in ionic liquids [J].

Kragl, U ;

Eckstein, M ;

Kaftzik, N .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (06) :565-571

[5]

Lee YM, 2007, J MICROBIOL BIOTECHN, V17, P600

[6] Stabilization of α-chymotrypsin by ionic liquids in transesterification reactions [J].

Lozano, P ;

de Diego, T ;

Guegan, JP ;

Vaultier, M ;

Iborra, JL .

BIOTECHNOLOGY AND BIOENGINEERING, 2001, 75 (05) :563-569

[7] Thermal stability and activity regain of horseradish peroxidase in aqueous mixtures of imidazolium-based ionic liquids [J].

Machado, MF ;

Saraiva, JM .

BIOTECHNOLOGY LETTERS, 2005, 27 (16) :1233-1239

[8] KINETICS OF HORSERADISH PEROXIDASE-CATALYZED POLYMERIZATION AND PRECIPITATION OF AQUEOUS 4-CHLOROPHENOL [J].

NICELL, JA .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1994, 60 (02) :203-215

[9]

Poker Y, 1987, BIOCHEMISTRY-US, V26, p[2597, 2606]

[10] SOLVATION EFFECTS UPON THE THERMODYNAMIC SUBSTRATE ACTIVITY - CORRELATION WITH THE KINETICS OF ENZYME CATALYZED-REACTIONS .2. MORE COMPLEX INTERACTIONS OF ALPHA-CHYMOTRYPSIN WITH DIOXANE AND ACETONE WHICH ARE ALSO COMPETITIVE INHIBITORS [J].

SMITH, RR ;

CANADY, WJ .

BIOPHYSICAL CHEMISTRY, 1992, 43 (02) :189-195

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