Native electron capture dissociation for the structural characterization of noncovalent interactions in native cytochrome c

被引:80
作者
Breuker, K
McLafferty, FW
机构
[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria
[2] Cornell Univ, Dept Chem & Chem Biol, Baker Lab, Ithaca, NY 14853 USA
关键词
cytochrome c; electron capture dissociation; electron transfer; mass spectrometry; noncovalent interactions;
D O I
10.1002/anie.200351705
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Selectively cleaved: Under appropriate experimental conditions, electrospray ionization mass spectrometry produces fragment ions that directly indicate non-covalent protein-heme interaction sites in native Cytochrome c (see picture). The proposed mechanism involves asymmetric charge partitioning in gaseous protein dimer ions, which causes electron transfer and subsequent backbone-cleavage.
引用
收藏
页码:4900 / 4904
页数:5
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