Pigment Epithelium-derived Factor Protects Retinal Pigment Epithelial Cells Against Cytotoxicity "In Vitro"

被引：15
|
作者：
Nadal-Nicolas, Francisco M. [1 ]
Becerra, S. Patricia [1 ]
机构：
[1] NEI, Sect Prot Struct & Funct, Lab Retinal Cell & Mol Biol, NIH, Bethesda, MD 20892 USA
来源：
RETINAL DEGENERATIVE DISEASES: MECHANISMS AND EXPERIMENTAL THERAPY | 2018年 / 1074卷
关键词：
Pigment epithelium-derived factor (PEDF); Retinal pigment epithelium (RPE); ARPE-19; cells; Toxicity; Oxidative stress; Sodium iodate (NaIO3); Viability; Age-related macular degeneration; OXIDATIVE STRESS; SODIUM-IODATE; INTERPHOTORECEPTOR MATRIX; PEDF; DEGENERATION; DYSFUNCTION; PREVALENCE; DAMAGE; DEATH;
D O I：
10.1007/978-3-319-75402-4_56
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Oxidative stress has been implicated in neurodegenerative diseases, such as age-related macular degeneration. Hydrogen peroxide and sodium iodate can mediate oxidative injury. Sodium iodate induces a selective retinal degeneration targeting the RPE. We describe a method of chronic sodium iodate-mediated injury on RPE cells that may serve to evaluate protective factors against oxidative stress. Cytotoxicity and cell viability curves of ARPE-19 cells with sodium iodate were generated. The antioxidant pigment epithelium-derived factor decreased sodium iodate-mediated cytotoxicity without affecting ARPE-19 cell viability. A cell culture system to evaluate protection against oxidative stress injury with PEDF is discussed.
引用
收藏
页码：457 / 464
页数：8
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