Pigment Epithelium-derived Factor Protects Retinal Pigment Epithelial Cells Against Cytotoxicity "In Vitro"

被引：15
|
作者：
Nadal-Nicolas, Francisco M. [1 ]
Becerra, S. Patricia [1 ]
机构：
[1] NEI, Sect Prot Struct & Funct, Lab Retinal Cell & Mol Biol, NIH, Bethesda, MD 20892 USA
来源：
RETINAL DEGENERATIVE DISEASES: MECHANISMS AND EXPERIMENTAL THERAPY | 2018年 / 1074卷
关键词：
Pigment epithelium-derived factor (PEDF); Retinal pigment epithelium (RPE); ARPE-19; cells; Toxicity; Oxidative stress; Sodium iodate (NaIO3); Viability; Age-related macular degeneration; OXIDATIVE STRESS; SODIUM-IODATE; INTERPHOTORECEPTOR MATRIX; PEDF; DEGENERATION; DYSFUNCTION; PREVALENCE; DAMAGE; DEATH;
D O I：
10.1007/978-3-319-75402-4_56
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Oxidative stress has been implicated in neurodegenerative diseases, such as age-related macular degeneration. Hydrogen peroxide and sodium iodate can mediate oxidative injury. Sodium iodate induces a selective retinal degeneration targeting the RPE. We describe a method of chronic sodium iodate-mediated injury on RPE cells that may serve to evaluate protective factors against oxidative stress. Cytotoxicity and cell viability curves of ARPE-19 cells with sodium iodate were generated. The antioxidant pigment epithelium-derived factor decreased sodium iodate-mediated cytotoxicity without affecting ARPE-19 cell viability. A cell culture system to evaluate protection against oxidative stress injury with PEDF is discussed.
引用
收藏
页码：457 / 464
页数：8
相关论文
共 50 条
  • [21] Pigment epithelium-derived factor (PEDF) in the monkey eye: Apical secretion from the retinal pigment epithelium.
    Becerra, SP
    Wu, YQ
    Montuenga, L
    Wong, P
    Pfeffer, B
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2001, 42 (04) : S772 - S772
  • [22] Pigment epithelium-derived factor protects cultured cerebellar granule cells against glutamate-induced neurotoxicity
    Taniwaki, T
    Hirashima, N
    Becerra, SP
    Chader, GJ
    Etcheberrigaray, R
    Schwartz, JP
    JOURNAL OF NEUROCHEMISTRY, 1997, 68 (01) : 26 - 32
  • [23] Pigment Epithelium-Derived Factor of Glial Origin enhances Survival of Retinal Ganglion Cells
    Unterlauft, Jan D.
    Savkovic-Cvijic, Helena
    Wiedemann, Peter M. H.
    Eichler, Wolfram
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2015, 56 (07)
  • [24] Pigment epithelium derived factor (PEDF), a candidate for autocrine regulation of retinal pigment epithelial cells.
    Feher, J
    Albedi, FM
    Formisano, G
    Perilli, R
    Scorcia, G
    Pintucci, S
    Caiazza, S
    TombranTink, J
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1997, 38 (04) : 2753 - 2753
  • [25] Pigment Epithelium-Derived Factor Secreted from Retinal Pigment Epithelium Facilitates Apoptotic Cell Death of iPSC
    Kanemura, Hoshimi
    Go, Masahiro J.
    Nishishita, Naoki
    Sakai, Noriko
    Kamao, Hiroyuki
    Sato, Yoji
    Takahashi, Masayo
    Kawamata, Shin
    SCIENTIFIC REPORTS, 2013, 3
  • [26] Pigment Epithelium-Derived Factor Secreted from Retinal Pigment Epithelium Facilitates Apoptotic Cell Death of iPSC
    Hoshimi Kanemura
    Masahiro J. Go
    Naoki Nishishita
    Noriko Sakai
    Hiroyuki Kamao
    Yoji Sato
    Masayo Takahashi
    Shin Kawamata
    Scientific Reports, 3
  • [27] Overexpression of Pigment Epithelium-Derived Factor Inhibits Retinal Inflammation and Neovascularization
    Park, Kyoungmin
    Jin, Ji
    Hu, Yang
    Zhou, Kevin
    Ma, Jian-xing
    AMERICAN JOURNAL OF PATHOLOGY, 2011, 178 (02): : 688 - 698
  • [28] Pigment Epithelium-Derived Factor Protects Retinal Neural Cells and Prevents Pathological Angiogenesis in an Ex Vivo Ischemia Model
    Xi, Lei
    Tikhonovich, Marina
    Biesemeier, Antje
    Julien-Schraermeyer, Sylvie
    Schraermeyer, Ulrich
    Tschulakow, Alexander V. V.
    OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2022, 2022
  • [29] EXPRESSION OF PIGMENT EPITHELIUM-DERIVED FACTOR IN WOUND HEALING CELLS
    Harries, R. L.
    Owen, S.
    Ruge, F.
    Cai, J.
    Torkington, J.
    Harding, K. G.
    Jiang, W.
    WOUND REPAIR AND REGENERATION, 2015, 23 (02) : A24 - A24
  • [30] EXPRESSION OF PIGMENT EPITHELIUM-DERIVED FACTOR IN WOUND HEALING CELLS
    Harries, Rhiannon L.
    Owen, Sioned
    Ruge, F.
    Cai, Jun
    Torkington, James
    Harding, Keith G.
    Jiang, Wen G.
    ANTICANCER RESEARCH, 2015, 35 (07) : 4366 - 4367
12345