Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock

被引:343
作者
Fowler, SG
Cook, D
Thomashow, ME [1 ]
机构
[1] Michigan State Univ, US Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA
关键词
D O I
10.1104/pp.104.058354
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Exposing Arabidopsis (Arabidopis thaliana) plants to low temperature results in rapid induction of CBF1, 2, and 3 (CBF1-3; also known as DREB1B, C, and A, respectively), which encode transcriptional activators that induce expression of a battery of genes that increase plant freezing and chilling, tolerance. Recently,,it has been shown that, basal levels of CBF3 transcripts and those of certain CBF-regulated genes exhibit circadian cycling. Here, we further explored the regulation of CBF1-3 by the circadian clock. The results indicated that the extent to which CBF1-3 transcripts accumulated in response to low temperature was dependent on the time of day that the plants were exposed to low temperature and that this was regulated by the circadian clock. The highest and lowest levels of cold-induced CBF1-3 transcript accumulation occurred at 4 and 16 h after subjective dawn, respectively. An analysis of CBF2. promoter-reporter gene fusions indicated that this control included transcriptional regulation. In addition, the cold responsiveness. of RAV1 and ZAT12, genes that are cold induced in parallel with CBF1-3, was also subject to circadian regulation. However, whereas the maximum level of cold-induced RAV1 transcript accumulation occurred at the same time of day as did CBF1-3 transcripts, that of ZAT12 Was in reverse phase, i.e. the highest level of cold-induced ZAT12 transcript accumulation occurred 16 h after subjective dawn. These results indicate that cold-induced expression of CBF1-3, RAV1, and 2 AT12 is gated by the circadian clock and suggest that this regulation likely occurs through at least two nonidentical (though potentially overlapping) signaling pathways.
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页码:961 / 968
页数:8
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共 45 条
[31]   CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in, stress tolerance in Arabidopsis [J].
Novillo, F ;
Alonso, JM ;
Ecker, JR ;
Salinas, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (11) :3985-3990
[32]  
Riechmann JL, 1998, BIOL CHEM, V379, P633
[33]   CORRELATION BETWEEN THE CIRCADIAN-RHYTHM OF RESISTANCE TO EXTREME TEMPERATURES AND CHANGES IN FATTY-ACID COMPOSITION IN COTTON SEEDLINGS [J].
RIKIN, A ;
DILLWITH, JW ;
BERGMAN, DK .
PLANT PHYSIOLOGY, 1993, 101 (01) :31-36
[34]   The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis [J].
Rizhsky, L ;
Davletova, S ;
Liang, HJ ;
Mittler, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (12) :11736-11743
[35]   The late elongated hypocotyl mutation of Arabidopsis disrupts circadian rhythms and the photoperiodic control of flowering [J].
Schaffer, R ;
Ramsay, N ;
Samach, A ;
Corden, S ;
Putterill, J ;
Carré, IA ;
Coupland, G .
CELL, 1998, 93 (07) :1219-1229
[36]   Plants in a cold climate [J].
Smallwood, M ;
Bowles, DJ .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2002, 357 (1423) :831-846
[37]  
Somers DE, 1998, DEVELOPMENT, V125, P485
[38]   Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit [J].
Stockinger, EJ ;
Gilmour, SJ ;
Thomashow, MF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (03) :1035-1040
[39]   Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms [J].
Thomashow, MF .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1999, 50 :571-599
[40]   So what's new in the field of plant cold acclimation? Lots! [J].
Thomashow, MF .
PLANT PHYSIOLOGY, 2001, 125 (01) :89-93