Single-particle tracking of quantum dot-conjugated prion proteins inside yeast cells

被引：14

作者：

Tsuji, Toshikazu ^{[1
]}

Kawai-Noma, Shigeko ^{[1
]}

Pack, Chan-Gi ^{[2
]}

Terajima, Hideki ^{[1
]}

Yajima, Junichiro ^{[3
]}

Nishizaka, Takayuki ^{[3
]}

Kinjo, Masataka ^{[4
]}

Taguchi, Hideki ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[2] RIKEN Adv Sci Inst, Cellular Informat Lab, Wako, Saitama 3510198, Japan

[3] Gakushuin Univ, Dept Phys, Toshima Ku, Tokyo 1718588, Japan

[4] Hokkaido Univ, Grad Sch Life Sci, Lab Mol Cell Dynam, Sapporo, Hokkaido 0010021, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 405卷 / 04期

关键词：

Quantum dots; Single-particle tracking; Yeast; Yeast prion proteins; CROSS-CORRELATION SPECTROSCOPY; LIVING CELLS; DYNAMICS; SUP35; TRANSMISSION; PROPAGATION; INHERITANCE; DIFFUSION; HSP104; FIBER;

D O I：

10.1016/j.bbrc.2011.01.083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast is a model eukaryote with a variety of biological resources. Here we developed a method to track a quantum dot (QD)-conjugated protein in the budding yeast Saccharomyces cerevisiae. We chemically Conjugated QDs with the yeast prion Sup35, incorporated them into yeast spheroplasts, and tracked the motions by conventional two-dimensional or three-dimensional tracking microscopy. The method paves the way toward the individual tracking of proteins of interest inside living yeast cells. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：638 / 643

页数：6

共 31 条

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