A rhamnan-type sulfated polysaccharide with novel structure from Monostroma angicava Kjellm (Chlorophyta) and its bioactivity

被引:49
作者
Liu, Xue [1 ]
Hao, Jiejie [1 ]
He, Xiaoxi [1 ]
Wang, Shuyao [1 ]
Cao, Sujian [1 ]
Qin, Ling [1 ]
Mao, Wenjun [1 ,2 ]
机构
[1] Ocean Univ China, Sch Med & Pharm, Shandong Prov Key Lab Glycosci & Glycotechnol, Lab Marine Drugs,Minist Educ, Qingdao 266003, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
基金
中国国家自然科学基金;
关键词
Monostroma angicava Kjellm; Sulfated polysaccharide; Structure; Antidiabetic activity; Anticoagulant property; ANTICOAGULANT ACTIVITY; SEQUENCE-ANALYSIS; OLIGOSACCHARIDES; ALGAE; ELUCIDATION; MECHANISMS; HEPARIN; CELLS;
D O I
10.1016/j.carbpol.2017.06.031
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A homogeneous polysaccharide was obtained from Monostroma angicava Kjellm by water extraction, preparative anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that the polysaccharide was a glucuronic acid-containing rhamnan-type sulfated polysaccharide. The backbone mainly consisted of -> 3)-alpha-L-Rhap-(1 -> and -> 2)-alpha-L-Rhap-(1 -> residues, partially sulfated at C-2 of -> 3)-alpha-LRhap-(1 -> and C-3/C-4 of -> 2)-alpha-L-Rhap The branching contained unsulfated or monosulfated 3-linked, 2-linked, 4-linked alpha-L-rhamnose and terminal beta-D-glucuronic acid residues. The polysaccharide had strong antidiabetic activity assessed by glucose consumption, total cholesterol and triglyceride levels using human hepatocellular carcinoma (HepG2) and insulin-resistant HepG2 cells. The polysaccharide exhibited high anticoagulant property by activated partial thromboplastin time and thrombin time assays, and possessed high fibrin(ogen)olytic activity evaluated by plasminogen activator inhibitior-1, fibrin(ogen) degradation products and D-dimer levels using rats plasma. The investigation demonstrated that the polysaccharide from Monostroma angicava Kjellm was a novel sulfated rhamnan and could be a potential antidiabetic and anticoagulant polysaccharide. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:732 / 748
页数:17
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