Ishige okamurae Ameliorates Methylglyoxal-Induced Nephrotoxicity via Reducing Oxidative Stress, RAGE Protein Expression, and Modulating MAPK, Nrf2/ARE Signaling Pathway in Mouse Glomerular Mesangial Cells

被引：13

作者：

Kim, Mingyeong ^{[1
]}

Cho, Chiheung ^{[2
]}

Lee, Changjun ^{[2
,3
]}

Ryu, Bomi ^{[4
]}

Kim, Sera ^{[2
]}

Hur, Jinyoung ^{[2
]}

Lee, Sang-Hoon ^{[1
,2
]}

机构：

[1] Univ Sci & Technol, Dept Food Biotechnol, Daejeon 34113, South Korea

[2] Korea Food Res Inst, Div Funct Food Res, 245 Nongsaengmyeong Ro, Iseo Myeon 55365, Wanju Gun, South Korea

[3] Chonbuk Natl Univ, Dept Food Sci & Technol, Jeonju 54896, South Korea

[4] Jeju Natl Univ, Inst Marine Sci, Jeju 63333, South Korea

来源：

FOODS | 2021年 / 10卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

Ishige okamurae; advanced glycation end-product; nephropathy; methylglyoxal; Nrf2/ARE pathway; ADVANCED GLYCATION ENDPRODUCTS; ENDOPLASMIC-RETICULUM STRESS; END-PRODUCTS; ANTIOXIDANT; HYPERGLYCEMIA; APOPTOSIS; KINASE;

D O I：

10.3390/foods10092000

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Advanced glycation end-products (AGEs) such as methylglyoxal (MGO) play a vital role in the pathogenesis of nephropathy, a diabetic complication. In the present study, we evaluated the anti-glycation and renal protective properties of Ishige okamurae extract (IOE) against AGE-induced oxidative stress. HPLC analysis confirmed that bioactive phlorotannins such as diphlorethohydroxycarmalol and ishophloroglucin A are predominantly present in IOE. IOE showed strong anti-glycation activities via inhibition of AGE formation, inhibition of AGE-protein cross-linking, and breaking of AGE-protein cross-links. In addition, in vitro studies using mesangial cells demonstrated that IOE effectively suppressed intracellular reactive oxygen species production, intracellular MGO accumulation, and apoptotic cell death by MGO-induced oxidative stress, in addition to regulating the expression of proteins involved in the receptor for AGEs and nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response elements (ARE) signaling pathways. Therefore, IOE can serve as a natural therapeutic agent for the management of AGE-related nephropathy.

引用

页数：15

共 31 条

[11] Diphlorethohydroxycarmalol isolated from Ishige okamurae, a brown algae, a potent α-glucosidase and α-amylase inhibitor, alleviates postprandial hyperglycemia in diabetic [J].