Effect of Different Aβ Aggregates as Antigen on the Measure of Naturally Occurring Autoantibodies against Amyloid-β40/42 in IVIG

被引：3

作者：

Cao, Haijun ^{[1
]}

Du, Xi ^{[1
]}

Zeng, Renyong ^{[1
]}

Lv, Zhaoji ^{[1
]}

Ye, Shengliang ^{[1
]}

Jiang, Peng ^{[1
]}

Wang, Zongkui ^{[1
]}

Ma, Li ^{[1
]}

Huang, Yun ^{[2
]}

Li, Changqing ^{[1
]}

Zhang, Rong ^{[1
]}

Liu, Fengjuan ^{[2
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Blood Transfus, 26 Huacai Rd, Chengdu 610052, Sichuan, Peoples R China

[2] Southwest Petr Univ, Sch Mat Sci & Engn, 8 Xindu Rd, Chengdu 610500, Sichuan, Peoples R China

来源：

CURRENT ALZHEIMER RESEARCH | 2019年 / 16卷 / 14期

关键词：

Naturally occurring autoantibodies against amyloid-beta; intravenous immunoglobulin; Alzheimer's disease; measure; amyloid beta-protein; aggregate; INTRAVENOUS IMMUNOGLOBULIN; ALZHEIMERS-DISEASE; PRECLINICAL MODELS; ANTIBODIES; OLIGOMERS; MONOMER; PEPTIDE; ELISA;

D O I：

10.2174/1567205017666200102151731

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Background: The specific Intravenous Immunoglobulin (IVIG) for Alzheimer's Disease (AD) is developing, which contains a high level of naturally occurring autoantibodies against amyloid-beta (nAbs-A beta), and the measure of nAbs-A beta content is greatly essential. Though Enzyme-Linked Immunosorbent Assay (ELISA) has been widely used in detecting the nAbs-A beta content, the impact of A beta aggregates species chosen as antigen in ELISA on this measure has not been evaluated. Objective: To clarify the influence of different A beta(40/42) aggregates as antigen during ELISA on the content of nAbs-A beta(40/42) measured in WIG. Method: Preparation of various A beta(40/42) aggregates was performed by different aggregation solutions and various lengths of time, and analyzed by western blot. Different A beta(40/42) aggregates as antigen were adopted to measure the nAbs-A beta(40/42) content in IVIG by ELISA, and the control was carried out to reduce interference of nonspecific binding. The Bonferroni and Dunnett's T3 were used for statistical analysis. Results: The duration for the formation of A beta(40/42) aggregates had more effect on detecting nAbs-A beta(40/42) content in IVIG than the aggregation solution. Higher content of nAbs-A beta(40/42) in the same IVIG was displayed when measured with A beta(40/42) aggregates at day 3, instead of at day 0.5 and day 7.0. The nAbs-A beta(40/42) contents in the same IVIG measured with A beta(40/42) aggregates prepared in different solutions were obviously different, but there was no significant regularity among them. Conclusion: The nAbs-A beta(40/42) content in the same EVIG is significantly different when measured with A beta(40/42) aggregated under different conditions. The nAbs-A beta(40/42)content in IVIG by antigen-dependent measures, like ELISA, is uncertain.

引用

页码：1290 / 1299

页数：10

共 38 条

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