Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona

被引：6
作者：
Fernandez-Naval, Candela [1 ]
Arando, Maider [2 ]
Espasa, Mateu [3 ]
Anton, Andres [1 ]
Fernandez-Huerta, Miguel [4 ]
Silgado, Aroa [1 ]
Pinatar, Cristina [1 ]
Zarzuela, Francesc [2 ]
Gonzalez-Lopez, Juan Jose [1 ]
Serra-Pladevall, Judit [1 ]
Sulleiro, Elena [1 ]
Pumarola, Tomas [1 ]
Vall-Mayans, Marti [5 ]
Esperalba, Juliana [1 ]
机构：
[1] Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Dept Microbiol, Vall dHebron Res Inst, Passeig Vall dHebron 119-129, Barcelona 08035, Spain
[2] Univ Autonoma Barcelona, Hosp Univ Vall dHebron, Sexually Transmitted Infect Unit Vall dHebron Dra, Carrer St Oleguer 17, Barcelona 08001, Spain
[3] Corp Sanitaria Parc Tauli, Dept Microbiol, Parc Tauli 1, Barcelona 08208, Spain
[4] Hosp Univ Bellvitge, Dept Microbiol, Barcelona 08907, Spain
[5] Hosp Germans Trias i Pujol, Dept Microbiol, Carretera Canyet, Barcelona 08916, Spain
来源：
FUTURE MICROBIOLOGY | 2021年 / 16卷 / 13期
关键词：
macrolide resistance; molecular epidemiology; molecular typing; multilocus sequence typing; phylogeny; syphilis; Treponema pallidum; MACROLIDE RESISTANCE; PCR TEST; SYPHILIS; MUTATION;
D O I：
10.2217/fmb-2021-0037
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe. Tweetable abstract We successfully identified 11 allelic profiles of T. pallidum from 63 fully typed syphilis samples using the MLST methodology. Profile 1.3.1 was found in 56% of samples, all of them being macrolide resistant.
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页码：967 / 976
页数：10
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