Measurement of Telomere Length Using PNA Probe by Cytometry

被引：5

作者：

Carbonari, Maurizio ^{[1
]}

Cibati, Marina ^{[1
]}

Sette, Nicla ^{[1
]}

Catizone, Angela ^{[2
]}

Fiorilli, Massimo ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Clin Med, Rome, Italy

[2] Univ Roma La Sapienza, Histol & Med Embryol Dept, Rome, Italy

来源：

RECENT ADVANCES IN CYTOMETRY, PART B: ADVANCES IN APPLICATIONS, FIFTH EDITION | 2011年 / 103卷

关键词：

IN-SITU HYBRIDIZATION; FLUORESCENT INSITU HYBRIDIZATION; CELL-SURFACE MARKERS; FLOW-CYTOMETRY; TERMINAL TRANSFERASE; HUMAN FIBROBLASTS; T-CELLS; DNA; RNA; IDENTIFICATION;

D O I：

10.1016/B978-0-12-385493-3.00008-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Peptide nucleic acid (PNA) probes hybridize to denatured telomeric sequences in cells permeabilized in hot formamide. In reported protocols, the hybridization was conducted in solutions with high formamide concentrations to avoid the DNA renaturation that can hamper binding of the oligo-PNA probe to specific sequences. We postulated that telomeric DNA, confined in the nuclear microvolume, is not able to properly renature after hot formamide denaturation. Therefore, to improve hybridization conditions between the probe and the target sequences, it might be possible to add probe to sample after the complete removal of formamide.

引用

页码：189 / 202

页数：14

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