Total phenolic contents, radical scavenging and cyclic voltammetry of seaweeds from Brittany

被引:66
作者
Keyrouz, R. [1 ]
Abasq, M. L. [1 ]
Le Bourvellec, C. [1 ]
Blanc, N. [1 ]
Audibert, L. [2 ]
ArGall, E. [2 ]
Hauchard, D. [1 ]
机构
[1] Univ Rennes 1, Ecole Natl Super Chim Rennes, CNRS, UMR 6226, F-35708 Rennes 7, France
[2] Univ Bretagne Occidentale UEB, Lab Ecophysiol & Biotechnol Halophytes & Algues M, EA 3877, IUEM, F-29280 Plouzane, Brittany, France
关键词
Antioxidant; Seaweed extracts; Folin-Glombitza method; DPPH; Cyclic voltammetry; PERFORMANCE LIQUID-CHROMATOGRAPHY; COULOMETRIC ARRAY DETECTION; ANTIOXIDANT ACTIVITY; CAPACITY; FLAVONOIDS; FRUITS; DAMAGE; ASSAY; FRAP; TEA;
D O I
10.1016/j.foodchem.2010.10.061
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The role of polyphenols as antioxidants in our daily nutrition has been extensively investigated, but information on composition of tannins from marine sources on nutrient utilisation is limited. This study will be focused on the determination of the polyphenol quantities in brown seaweeds from Brittany coast in France; the extracts were delivered from algae centre for valorisation (CEVA), and the fidelity of the method was examined intraday and interlaboratories. The seaweed's extracts are divided into three categories: Fucus Vesiculosus, Ascophyllum nodosum and Fucus Serratus. Consequently, the total polyphenol contents (TPC) of these extracts will be measured using Folin-Glombitza spectrophotometric method. Thereafter, the antioxidant properties of these polyphenols were determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. All polyphenols extracted were tested using cyclic voltammetry (CV) in aprotic media. The CV is realised to compare the results from spectroscopic method and to electrochemically characterise the seaweed's polyphenols. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:831 / 836
页数:6
相关论文
共 24 条
[1]   Analysis of flavonoids and other phenolic compounds using high-performance liquid chromatography with coulometric array detection: Relationship to antioxidant activity [J].
Aaby, K ;
Hvattum, E ;
Skrede, G .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (15) :4595-4603
[2]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[3]   Electrochemical approach for discriminating and measuring predominant flavonoids and phenolic acids using differential pulse voltammetry:: towards an electrochemical index of natural antioxidants [J].
Blasco, AJ ;
González, MC ;
Escarpa, A .
ANALYTICA CHIMICA ACTA, 2004, 511 (01) :71-81
[4]   ANTIOXIDANT DETERMINATIONS BY THE USE OF A STABLE FREE RADICAL [J].
BLOIS, MS .
NATURE, 1958, 181 (4617) :1199-1200
[5]   OXYGEN-RADICAL ABSORBENCY CAPACITY ASSAY FOR ANTIOXIDANTS [J].
CAO, GH ;
ALESSIO, HM ;
CUTLER, RG .
FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (03) :303-311
[6]   Antioxidant capacity of tea and common vegetables [J].
Cao, GH ;
Sofic, E ;
Prior, RL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (11) :3426-3431
[7]   Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay [J].
Guo, CJ ;
Yang, JJ ;
Wei, JY ;
Li, YF ;
Xu, J ;
Jiang, YG .
NUTRITION RESEARCH, 2003, 23 (12) :1719-1726
[8]   High-performance liquid chromatography coupled with coulometric array detection of electroactive components in fruits and vegetables: Relationship to oxygen radical absorbance capacity [J].
Guo, CJ ;
Cao, GH ;
Sofic, E ;
Prior, RL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (05) :1787-1796
[9]  
Halliwell B, 1996, ANNU REV NUTR, V16, P33, DOI 10.1146/annurev.nu.16.070196.000341
[10]  
Halliwell Barry, 1999, Food Science and Agricultural Chemistry, V1, P67