Immobilization of active lipase B from Candida antarctica on the surface of polyhydroxyalkanoate inclusions

被引：16

作者：

Jahns, Anika C. ^{[1
,2
]}

Rehm, Bernd H. A. ^{[1
,2
,3
]}

机构：

[1] Massey Univ, Inst Fundamental Sci, Palmerston North 4442, New Zealand

[2] PolyBatics Ltd, BioCommerce Ctr, Palmerston North 4442, New Zealand

[3] MacDiarmid Inst Adv Mat & Nanotechnol, Wellington 6140, New Zealand

来源：

BIOTECHNOLOGY LETTERS | 2015年 / 37卷 / 04期

关键词：

Beads; Lipase; PHA synthase (PhaC); Polyhydroxyalkanoate beads; POLYESTER SYNTHASES; MICROBIAL LIPASES; CATALYSTS; BEADS;

D O I：

10.1007/s10529-014-1735-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fusion of the PHA synthase, PhaC, to lipase B yielded active PHA lipase beads capable of hydrolyzing glycerol tributyrate. Lipase B beads showed stable activity over several weeks and re-usability without loss of function.

引用

页码：831 / 835

页数：5

共 14 条

[1] Candida antarctica lipase B chemically immobilized on epoxy-activated micro- and nanobeads:: Catalysts for polyester synthesis [J].

Chen, Bo ;

Hu, Jun ;

Miller, Elizabeth M. ;

Xie, Wenchun ;

Cai, Minmin ;

Gross, Richard A. .

BIOMACROMOLECULES, 2008, 9 (02) :463-471

[2]

Goodman LP, 1969, LIPIDS, V5, P362

[3] Bacterial Polyhydroxyalkanoate Granules: Biogenesis, Structure, and Potential Use as Nano-/Micro-Beads in Biotechnological and Biomedical Applications [J].

Grage, Katrin ;

Jahns, Anika C. ;

Parlane, Natalie ;

Palanisamy, Rajasekaran ;

Rasiah, Indira A. ;

Atwood, Jane A. ;

Rehm, Bernd H. A. .

BIOMACROMOLECULES, 2009, 10 (04) :660-669

[4] Microbial lipases form versatile tools for biotechnology [J].

Jaeger, KE ;

Reetz, MT .

TRENDS IN BIOTECHNOLOGY, 1998, 16 (09) :396-403

[5] Multifunctional Inorganic-Binding Beads Self-Assembled Inside Engineered Bacteria [J].

Jahns, Anika C. ;

Haverkamp, Richard G. ;

Rehm, Bernd H. A. .

BIOCONJUGATE CHEMISTRY, 2008, 19 (10) :2072-2080

[6] Improvement of enzyme activity, stability and selectivity via immobilization techniques [J].

Mateo, Cesar ;

Palomo, Jose M. ;

Fernandez-Lorente, Gloria ;

Guisan, Jose M. ;

Fernandez-Lafuente, Roberto .

ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (06) :1451-1463

[7] Imaging the distribution and secondary structure of immobilized enzymes using infrared microspectroscopy [J].

Mei, Y ;

Miller, L ;

Gao, W ;

Gross, RA .

BIOMACROMOLECULES, 2003, 4 (01) :70-74

[8] A novel alkaline lipase from Burkholderia cepacia for detergent formulation [J].

Rathi, P ;

Saxena, RK ;

Gupta, R .

PROCESS BIOCHEMISTRY, 2001, 37 (02) :187-192

[9] Genetics and biochemistry of polyhydroxyalkanoate granule self-assembly: The key role of polyester synthases [J].

Rehm, BHA .

BIOTECHNOLOGY LETTERS, 2006, 28 (04) :207-213

[10] Polyester synthases: natural catalysts for plastics [J].

Rehm, BHA .

BIOCHEMICAL JOURNAL, 2003, 376 :15-33

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