EIS microfluidic chips for flow immunoassay and ultrasensitive cholera toxin detection

被引:51
作者
Chiriaco, Maria Serena [1 ,2 ]
Primiceri, Elisabetta [1 ,2 ]
D'Amone, Eliana [1 ]
Ionescu, Rodica Elena [2 ]
Rinaldi, Ross [1 ,3 ]
Maruccio, Giuseppe [1 ,4 ]
机构
[1] NNL CNR Ist Nanosci, Lecce, Italy
[2] Univ Salento, Scuola Super ISUFI, Lecce, Italy
[3] Univ Salento, Dept Innovat Engn, Lecce, Italy
[4] Univ Salento, Dept Phys, Lecce, Italy
关键词
ON-A-CHIP; IMPEDANCE SPECTROSCOPY; ANTIBODY MICROARRAY; ARRAY BIOSENSOR; LIPID-MEMBRANE; ELECTRODE; SENSORS; IMMUNOSENSOR; CYTOTOXICITY; DIAGNOSTICS;
D O I
10.1039/c0lc00409j
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A flow-injection impedimetric immunosensor for the sensitive, direct and label-free detection of cholera toxin is reported. Alimit of detection smaller than 10 pM was achieved, a value thousands of times lower than the lethal dose. The developed chips fulfil the requirement of low cost and quick reply of the assay and are expected to enable field screening, prompt diagnosis and medical intervention without the need of specialized personnel and expensive equipment, a perspective of special relevance for use in developing countries. Since the chip layout includes two sensing areas each one with a 2 x 2 sensor array, our biochips can allow statistical or (alternatively) multiplex analysis of biorecognition events between antibodies immobilized on each working electrode and different antigens flowing into the chamber.
引用
收藏
页码:658 / 663
页数:6
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