Regeneration of fertile plants from cryopreserved cell suspensions of Arabidopsis thaliana (L) Heynh

被引：10
|
作者：
Ribeiro, RCS
Jekkel, Z
Mulligan, BJ
Cocking, EC
Power, JB
Davey, MR
Lynch, PT
机构：
[1] UNIV NOTTINGHAM,DEPT LIFE SCI,PLANT GENET MANIPULAT GRP,NOTTINGHAM NG7 2RD,ENGLAND
[2] UNIV AGR SCI,DEPT GENET & PLANT BREEDING,H-2103 GODOLLO,HUNGARY
来源：
PLANT SCIENCE | 1996年 / 115卷 / 01期
关键词：
Arabidopsis thaliana; cryopreservation; cell suspensions; pre-growth; plant regeneration;
D O I：
10.1016/0168-9452(96)04355-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Suspension cultured cells of the three ecotypes Landsberg Erecta, C24, and Wassilewskija of Arabidopsis thaliana were recovered from cryogenic storage. The post-thaw viability of cells, as determined by the reduction of triphenol tetrazolium chloride and cell re-growth, were influenced by pre-growth mannitol treatment. Fertile plants were rapidly regenerated from thawed cells.
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页码：115 / 121
页数：7
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