Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia

被引：7
作者：
Schroeck, Carmen [1 ]
Eydt, Carina [2 ]
Geburek, Florian [3 ]
Kaiser, Lena [1 ]
Paebst, Felicitas [4 ]
Burk, Janina [4 ,5 ]
Pfarrer, Christiane [2 ]
Staszyk, Carsten [1 ]
机构：
[1] Justus Liebig Univ, Inst Vet Anat Histol & Embryol, Giessen, Germany
[2] Univ Vet Med Hannover, Inst Anat, Hannover, Germany
[3] Justus Liebig Univ, Clin Horses, Giessen, Germany
[4] Univ Leipzig, Translat Ctr Regenerat Med TRM, Leipzig, Germany
[5] Univ Leipzig, Fac Vet Med, Large Anim Clin Surg, Leipzig, Germany
来源：
VETERINARY MEDICINE AND SCIENCE | 2017年 / 3卷 / 04期
关键词：
equine stem cells; flow cytometry; horse; proliferation assay; regenerative therapy; STEM-CELLS; AMNIOTIC-FLUID; ASPIRATION; TENDON;
D O I：
10.1002/vms3.74
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Allogeneic equine multipotent mesenchymal stromal cells (eMSCs) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSCs might be the bone marrow from euthanized horses. The purpose of this study was to compare invitro characteristics of equine bone marrow derived eMSC (eBM-MSCs) from euthanized horses (eut-MSCs) and from narcotized horses (nar-MSCs). Eut-MSCs and nar-MSCs showed typical eMSC marker profiles (positive: CD44, CD90; negative: CD11a/CD18 and MHCII) and possessed tri-lineage differentiation characteristics. Although CD105 and MHCI expression varied, no differences were detected between eut-MSCs and nar-MSCs. Proliferation characteristics did not differ between eut-MSCs and nar-MSCs, but age dependent decrease in proliferation and increase in MHCI expression was detected. These results suggest the possible use of eut-MSCs for therapeutic applications and production of commercial available eBM-MSC products.
引用
收藏
页码：239 / 251
页数：13
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