Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia

被引：7

作者：

Schroeck, Carmen ^{[1
]}

Eydt, Carina ^{[2
]}

Geburek, Florian ^{[3
]}

Kaiser, Lena ^{[1
]}

Paebst, Felicitas ^{[4
]}

Burk, Janina ^{[4
,5
]}

Pfarrer, Christiane ^{[2
]}

Staszyk, Carsten ^{[1
]}

机构：

[1] Justus Liebig Univ, Inst Vet Anat Histol & Embryol, Giessen, Germany

[2] Univ Vet Med Hannover, Inst Anat, Hannover, Germany

[3] Justus Liebig Univ, Clin Horses, Giessen, Germany

[4] Univ Leipzig, Translat Ctr Regenerat Med TRM, Leipzig, Germany

[5] Univ Leipzig, Fac Vet Med, Large Anim Clin Surg, Leipzig, Germany

来源：

VETERINARY MEDICINE AND SCIENCE | 2017年 / 3卷 / 04期

关键词：

equine stem cells; flow cytometry; horse; proliferation assay; regenerative therapy; STEM-CELLS; AMNIOTIC-FLUID; ASPIRATION; TENDON;

D O I：

10.1002/vms3.74

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Allogeneic equine multipotent mesenchymal stromal cells (eMSCs) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSCs might be the bone marrow from euthanized horses. The purpose of this study was to compare invitro characteristics of equine bone marrow derived eMSC (eBM-MSCs) from euthanized horses (eut-MSCs) and from narcotized horses (nar-MSCs). Eut-MSCs and nar-MSCs showed typical eMSC marker profiles (positive: CD44, CD90; negative: CD11a/CD18 and MHCII) and possessed tri-lineage differentiation characteristics. Although CD105 and MHCI expression varied, no differences were detected between eut-MSCs and nar-MSCs. Proliferation characteristics did not differ between eut-MSCs and nar-MSCs, but age dependent decrease in proliferation and increase in MHCI expression was detected. These results suggest the possible use of eut-MSCs for therapeutic applications and production of commercial available eBM-MSC products.

引用

页码：239 / 251

页数：13

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