Expression of p75NTR and its associated protein NADE in the rat cochlea

Objectives/Hypothesis: To investigate the expression of the low-affinity neurotrophin receptor p75 (p75NTR) and its associated protein NADE in the cochlea of the developing and the adult rat, Studies such as this one will help to predict the functional role of p75NTR and NADE in cochlear development, Study Design: Histochemical evaluation of p75NTR and NADE in the rat cochlea was performed. Methods: Immunohistochemical analysis was used to localize p75NTR and NADE in the rat cochlea at postnatal (PN) days PN0, PN2, PN4, PN6, PN8, PN10, and PN13 and in the adult. Confocal laser scanning microscopy was used to analyze whole-mount specimens. Results: Immunoreactivity of both p75NTR and NADE was observed in pillar cells. However, these proteins displayed reciprocal expression patterns. Expression of p75NTR was detected at PN0 and PN2, but disappeared after PN4, In contrast, NADE expression was initially detected at PN2 and persisted into adulthood. Conclusions: The neurotrophin receptor p75NTR and NADE have distinct and independent roles in developing and mature cochlea.