Non-thermal effects of microwaves on protease-catalyzed esterification and transesterification

被引：72

作者：

Roy, I ^{[1
]}

Gupta, MN ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, New Delhi 110016, India

来源：

TETRAHEDRON | 2003年 / 59卷 / 29期

关键词：

alpha-chymotrypsin; microwave-assisted reaction; non-aqueous enzymology; subtilisin; transesterification;

D O I：

10.1016/S0040-4020(03)00867-6

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The non-thermal effects of microwave irradiation on enzyme-catalyzed reactions have been evaluated by keeping the reaction temperature constant during irradiation. Subtilisin-catalyzed transesterification and alpha-chymotrypsin-catalyzed esterification have been carried out in six solvents of differing polarities and at three different temperatures. In all cases, microwave irradiation was found to increase the initial reaction rates by 2.1-4.7 times at all hydration levels. It is also shown that microwave irradiation can be used in conjunction with other strategies (like pH tuning and salt activation) for enhancing initial reaction rates. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：5431 / 5436

页数：6