Acetylcholine inhibits cell cycle progression in rat Schwann cells by activation of the M2 receptor subtype

被引:38
作者
Loreti, Simona [1 ]
Ricordy, Ruggero [2 ]
De Stefano, M. Egle [1 ]
Augusti-Tocco, Gabriella [1 ]
Tata, Ada Maria [1 ]
机构
[1] Univ Roma La Sapienza, Dept Cell & Dev Biol, Res Ctr Neurosci Daniel Bovet, Rome, Italy
[2] CNR, Inst Biol & Mol Pathol, Rome, Italy
关键词
muscarinic receptors; proliferation; neurotransmitter; axon-glia interaction; sciatic nerve;
D O I
10.1017/S1740925X08000045
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Cultures of schwalla cells froill ncoaatal rat sciatic aenes ivere treated with acety1cholilic agonists tvid the cjj cts oil cell Prolificratiou cniluatcd. 3[HI-thyaiidiac incorporation shows that accty1choliac (ACh) receptor agoiiists ilillibit cell proliftratioti, aiid FACS analysis deinoustratcs ccll-cyclc arrest and accunlulatioii of cells ill the Gi phase. The use of arecaiditie, a selective agouist of niuscariiiic M2 receptors reveals that this effect depends nictitily oil jW2 receptor acth,atioii. The arecaidine i1cpetialci i t- block ill Gi is rci,ersible becausc reinoval of arecaidiue froai the culture iiieditaii mduccs progrcssioil to the S phase. The block of the Gj-S transitioil is also characterized by inodulatiou of the expressioti ofscwrol cell-cycic inarkcrs. Morcoi,cr, trcatnicat ivith ACh receptor agmist causes both a decreasc ill the PCNA prolcin lci,cls ill Schivaiiii cell iniclci aad all iiicreasc ill P27 Olld P53 proteins. Filially, iinnnuio-elcctrou inicroscopy dcalonStl-atCS that M2 receptors arc expressed by Schivanii cells ill i,ivo. These results inclicate that ACh, by aiodulatiiig Schwaiin cell prolificratioii through M2 I-CCCPtOl- aclivatioa,)iiight cowributc to their progression to a niore dif rewiated phenotype.
引用
收藏
页码:269 / 279
页数:11
相关论文
共 53 条
[1]   Tripartite synapses: glia, the unacknowledged partner [J].
Araque, A ;
Parpura, V ;
Sanzgiri, RP ;
Haydon, PG .
TRENDS IN NEUROSCIENCES, 1999, 22 (05) :208-215
[2]   Cell cycle inhibitors p21 and p16 are required for the regulation of Schwann cell proliferation [J].
Atanasoski, S ;
Boller, D ;
De Ventura, L ;
Koegel, H ;
Boentert, M ;
Young, P ;
Werner, S ;
Suter, U .
GLIA, 2006, 53 (02) :147-157
[3]  
Bernardini N, 1999, J PERIPHER NERV SYST, V4, P222
[4]   Detection of basal and potassium-evoked acetylcholine release from embryonic DRG explants [J].
Bernardini, N ;
Tomassy, GS ;
Tata, AM ;
Augusti-Tocco, G ;
Biagioni, S .
JOURNAL OF NEUROCHEMISTRY, 2004, 88 (06) :1533-1539
[5]  
Biagioni S, 2000, INT J DEV BIOL, V44, P689
[6]   Selective allosteric enhancement of the binding and actions of acetylcholine at muscarinic receptor subtypes [J].
Birdsall, NJM ;
Farries, T ;
Gharagozloo, P ;
Kobayashi, S ;
Kuonen, D ;
Lazareno, S ;
Popham, A ;
Sugimoto, M .
LIFE SCIENCES, 1997, 60 (13-14) :1047-1052
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   Endothelins control the timing of Schwann cell generation in vitro and in vivo [J].
Brennan, A ;
Dean, CH ;
Zhang, AL ;
Cass, DT ;
Mirsky, R ;
Jessen, KR .
DEVELOPMENTAL BIOLOGY, 2000, 227 (02) :545-557
[9]  
BROCKES JP, 1987, METHOD ENZYMOL, V147, P217
[10]   Negative regulation of central nervous system myelination by polysialylated-neural cell adhesion molecule [J].
Charles, P ;
Hernandez, MP ;
Stankoff, B ;
Aigrot, MS ;
Colin, C ;
Rougon, G ;
Zalc, B ;
Lubetzki, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) :7585-7590