Modifying secretion and post-translational processing in insect cells

被引：104

作者：

Ailor, E ^{[1
]}

Betenbaugh, MJ ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Chem Engn, Baltimore, MD 21218 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 02期

关键词：

D O I：

10.1016/S0958-1669(99)80024-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.

引用

页码：142 / 145

页数：4

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