Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

被引：65

作者：

Andronov, E. E. ^{[1
]}

Petrova, S. N. ^{[2
]}

Pinaev, A. G. ^{[1
]}

Pershina, E. V. ^{[1
]}

Rakhimgalieva, S. Zh. ^{[3
]}

Akhmedenov, K. M. ^{[3
]}

Gorobets, A. V. ^{[4
]}

Sergaliev, N. Kh. ^{[3
]}

机构：

[1] Russian Acad Agr Sci, All Russia Res Inst Agr Microbiol VNIISkhM, St Petersburg 198608, Russia

[2] Orel State Agrarian Univ, Oryol 302019, Russia

[3] W Kazakhstan Agrarian Tech Univ, Minist Educ & Sci Republ Kazakhstan, Uralsk 090009, Kazakhstan

[4] Russian Acad Agr Sci, Dokuchaev Soil Sci Inst, Moscow 119017, Russia

来源：

EURASIAN SOIL SCIENCE | 2012年 / 45卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

DIVERSITY; BACTERIAL; METAGENOMICS; PATTERNS; GRADIENT; DNA;

D O I：

10.1134/S1064229312020044

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Molecular methods were used to study variation in the taxonomic structure of bacterial, archaeal, and fungal communities in soil samples taken along a salinity gradient from a solonchak in the vicinity of Lake Akkol' (Shingirlau, Kazakhstan). Soils from arable fields located 195 km from the solonchak served as the control. Total DNA was isolated from every sample and analyzed by T-RFLP and real-time PCR. Salinization was found to be the main ecological factor determining the structure of soil microbial community in the study region. The values of Simpson's index characterizing the diversity of this community proved to be similar in all the samples, which, however, significantly differed in the taxonomic composition of microorganisms. A significantly increased content of archaea was revealed in the sample with the highest salinity. The results of this study show that the structure of soil microbial community reflects specific features of a given soil and can be used as an indicator of its ecological state.

引用

页码：147 / 156

页数：10

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