Targeted delivery of mannan-coated superparamagnetic iron oxide nanoparticles to antigen-presenting cells for magnetic resonance-based diagnosis of metastatic lymph nodes in vivo

被引：50

作者：

Hieu Vu-Quang ^{[3
,4
]}

Yoo, Mi-Kyong ^{[1
,2
]}

Jeong, Hwan-Jeong ^{[5
]}

Lee, Hwa-Jeong ^{[3
,4
]}

Muthiah, Muthunarayanan ^{[3
,4
]}

Rhee, Joon Haeng ^{[4
]}

Lee, Jae-Hyuk ^{[6
]}

Cho, Chong-Su ^{[1
,2
]}

Jeong, Yong Yeon ^{[7
]}

Park, In-Kyu ^{[3
,4
]}

机构：

[1] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151921, South Korea

[2] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea

[3] Chonnam Natl Univ, Dept Biomed Sci, Res Inst Med Sci, Sch Med, Kwangju 501746, South Korea

[4] Chonnam Natl Univ, Clin Vaccine R&D Ctr, Hwasun Hosp, Jeonnam 519809, South Korea

[5] Chonbuk Natl Univ, Dept Nucl Med, Res Inst Clin Med, Med Sch & Hosp, Jeonju 561712, South Korea

[6] Chonnam Natl Univ, Dept Pathol, Hwasun Hosp, Jeonnam 519809, South Korea

[7] Chonnam Natl Univ, Dept Radiol, Hwasun Hosp, Jeonnam 519809, South Korea

来源：

ACTA BIOMATERIALIA | 2011年 / 7卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

Antigen-presenting cells; Metastatic lymph node; Magnetic resonance imaging; Mannan; Iron oxide nanoparticles; MR CONTRAST AGENTS; CENTRAL-NERVOUS-SYSTEM; BIOMEDICAL APPLICATIONS; MANNOSE RECEPTOR; PROSTATE-CANCER; FERUMOXIDES; MACROPHAGES; EXPERIENCE; PARTICLES; SAFETY;

D O I：

10.1016/j.actbio.2011.06.044

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Metastatic lymph nodes (LN) originate from primary cancer cells that metastasize to the lymphatic system. It is difficult to non-invasively discriminate between metastatic LN and normal LN because of their similarities in size and shape. Magnetic resonance (MR) contrast agents are widely utilized to enhance the image contrast among different tissues. Currently available dextran-based contrast agents are non-specifically internalized by macrophages. Therefore, the aim of this study was to develop mannan-coated superparamagnetic iron oxide nanoparticles (mannan-SPION) for specific delivery to immune cells in LN by receptor-mediated endocytosis for facilitated uptake in the target cells and faster acquisition of MR images. Mannan is a water soluble polysaccharide with a high content of D-mannose residues that can be recognized by mannose receptors on activated macrophages and dendritic cells. Mannan-SPION are proven to be suitable for MRI due to their small size, excellent aqueous stability, and lower cytotoxicity. Mannan-SPION are taken up by antigen-presenting cells such as macrophages and dendritic cells, which could be confirmed by Prussian blue and fluorescent staining. In addition, mannan-SPION exhibit enhanced delivery efficiency in targeting macrophages in LN in vivo compared with polyvinyl alcohol (PVA)-SPION. More specifically, the enhancement of MRI of LN by mannan-SPION increased dramatically during the earlier stages after intravenous injection, compared with PVA-SPION as a control, which indicates the potential for successful and early detection of metastastatic LN. Crown Copyright (C) 2011 Published by Elsevier Ltd. on behalf of Acta Materialia Inc. All rights reserved.

引用

页码：3935 / 3945

页数：11

共 34 条

[1] Use of ultrasmall superparamagnetic iron oxide in lymph node MR imaging in prostate cancer patients [J].

Barentsz, Jelle O. ;

Futterer, Jurgen J. ;

Takahashi, Satoru .

EUROPEAN JOURNAL OF RADIOLOGY, 2007, 63 (03) :369-372

[2]

BEAT AI, 2004, NAT REV IMMUNOL, V4, P432

[3] Iron oxide-enhanced MR lymphography: initial experience [J].

Bellin, MF ;

Beigelman, C ;

Precetti-Morel, S .

EUROPEAN JOURNAL OF RADIOLOGY, 2000, 34 (03) :257-264

[4] LIVER METASTASES - SAFETY AND EFFICACY OF DETECTION WITH SUPERPARAMAGNETIC IRON-OXIDE IN MR-IMAGING [J].

BELLIN, MF ;

ZAIM, S ;

AUBERTON, E ;

SARFATI, G ;

DURON, JJ ;

KHAYAT, D ;

GRELLET, J .

RADIOLOGY, 1994, 193 (03) :657-663

[5] Lymph node metastases: Safety and effectiveness of MR imaging with ultrasmall superparamagnetic iron oxide particles - Initial clinical experience [J].

Bellin, MF ;

Roy, C ;

Kinkel, K ;

Thoumas, D ;

Zaim, S ;

Vanel, D ;

Tuchmann, C ;

Richard, F ;

Jacqmin, D ;

Delcourt, A ;

Challier, E ;

Lebret, T ;

Cluzel, P .

RADIOLOGY, 1998, 207 (03) :799-808

[6] Superparamagnetic agents in magnetic resonance imaging: Physicochemical characteristics and clinical applications - A review [J].

Bonnemain, B .

JOURNAL OF DRUG TARGETING, 1998, 6 (03) :167-174

[7] The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation [J].

Burgdorf, Sven ;

Lukacs-Kornek, Veronika ;

Kurts, Christian .

JOURNAL OF IMMUNOLOGY, 2006, 176 (11) :6770-6776

[8] SUPERPARAMAGNETIC IRON-OXIDES AS POSITIVE MR CONTRAST AGENTS - INVITRO AND INVIVO EVIDENCE [J].

CHAMBON, C ;

CLEMENT, O ;

LEBLANCHE, A ;

SCHOUMANCLAEYS, E ;

FRIJA, G .

MAGNETIC RESONANCE IMAGING, 1993, 11 (04) :509-519

[9] Particle size investigations of a multistep synthesis of PVA coated superparamagnetic nanoparticles [J].

Chastellain, A ;

Petri, A ;

Hofmann, H .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2004, 278 (02) :353-360

[10] Macrophage imaging in central nervous system and in carotid atherosclerotic plaque using ultrasmall superparamagnetic iron oxide in magnetic resonance imaging [J].

Corot, C ;

Petry, KG ;

Trivedi, R ;

Saleh, A ;

Jonkmanns, C ;

Le Bas, JF ;

Blezer, E ;

Rausch, M ;

Brochet, B ;

Foster-Gareau, P ;

Balériaux, D ;

Gaillard, S ;

Dousset, V .

INVESTIGATIVE RADIOLOGY, 2004, 39 (10) :619-625

← 1 2 3 4 →