Enhanced production of Monacrosporium thaumasium protease and destruction action on root-knot nematode Meloidogyne java']javanica eggs

被引：6

作者：

Gouveia, Angelica de Souza ^{[1
]}

de Freitas Soares, Filippe Elias ^{[1
]}

Morgan, Tulio ^{[1
]}

Sufiate, Bruna Leite ^{[1
]}

Tavares, Gabriella Peterllini ^{[1
]}

Braga, Fabio Ribeiro ^{[3
]}

Avelar Monteiro, Thalita Suelen ^{[4
]}

Andre Genier, Hugo Leonardo ^{[2
]}

de Freitas, Leandro Grassi ^{[4
]}

de Queiroz, Jose Humberto ^{[1
]}

机构：

[1] Univ Fed Vicosa, Dept Biochem & Mol Biol, Vicosa, MG, Brazil

[2] Univ Fed Vicosa, Inst Biotechnol Appl Agr BIOAGRO, Dept Microbiol, Vicosa, MG, Brazil

[3] Univ Vila Velha, Vila Velha, ES, Brazil

[4] Univ Fed Vicosa, Dept Phytopatol, Vicosa, MG, Brazil

来源：

RHIZOSPHERE | 2017年 / 3卷

关键词：

Meloidogyne [!text type='java']java[!/text]nica; Monacrosporium thaumasium; Nematicidal effect; Nematophagous fungi; Protease; Solid state fermentation;

D O I：

10.1016/j.rhisph.2016.12.001

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nematophagous fungi, such as Monacrosporium thaumasium (NF34), have a high potential to produce enzymatic pool containing protease with nematicidal activity. Thus, the aim of this study was to optimize the conditions that affect this pool production by the fungus M. thaumasium on solid medium and its nematicidal effect on Meloidogyne javanica eggs. With the optimization assays, there was a significant increase in enzyme production, allowing a 30% reduction in larvae (p < 0.01) in the group treated with the ultrafiltration sample. The test of inhibitors was possible to classify it as a serine protease. This study determined the characteristics that allow the fungus M. thaumasium to produce a high level of protease with destruction effect on M. javanica eggs.

引用

页码：13 / 15

页数：3

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