Detection of specific antibodies anti-Neospora caninum in the fallow deer (Dama dama)

被引：10

作者：

Bien, Justyna ^{[1
]}

Moskwa, Bozena ^{[1
]}

Bogdaszewski, Marek ^{[1
]}

Cabaj, Wladyslaw ^{[1
]}

机构：

[1] Polish Acad Sci, Witold Stefanski Inst Parasitol, PL-00818 Warsaw, Poland

来源：

RESEARCH IN VETERINARY SCIENCE | 2012年 / 92卷 / 01期

关键词：

Fallow deer (Dama dama); Neospora caninum; ELISA; Western blot; Poland; WESTERN-BLOT-ANALYSIS; TOXOPLASMA-GONDII; TRICHINELLA IGG; WILD RUMINANTS; SEROPREVALENCE; ELISA; PREVALENCE;

D O I：

10.1016/j.rvsc.2010.10.014

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Sera from 335 farmed fallow deer (Dama dama) at the breeding station in Kosewo Game in the Mazurian Lake District, North-East Poland, were investigated for the presence of antibodies against Neospora caninum. The distribution of age groups was as follow: >4 years - 154 animals; 2 years - 76 animals; 1 year - 105 animals. Ten sera with the optical density exceeding 0.159 absorbance units (i.e., cut-off value) in ELISA test were also analyzed by Western blot. Western blot analysis revealed seroreactivity against immunodominant N. caninum antigens of 37, 25, and 16 kDa; however, in some sera additional bands were also visible. This is the first screening studies for antibodies against N. caninum in farmed fallow deer in Poland, in the region where neosporosis was confirmed in cattle and in farmed and free-ranging European red deer (Cervus elaphus). (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：96 / 98

页数：3

共 21 条

[1] Seroprevalence of Neospora caninum in non-carnivorous wildlife from Spain [J].

Almeria, S. ;

Vidal, D. ;

Ferrer, D. ;

Pabon, M. ;

Fernandez-de-Mera, M. I. G. ;

Ruiz-Fons, F. ;

Alzaga, V. ;

Marco, I. ;

Calvete, C. ;

Lavin, S. ;

Gortazar, C. ;

Lopez-Gatius, F. ;

Dubey, J. P. .

VETERINARY PARASITOLOGY, 2007, 143 (01) :21-28

[2] Neospora caninum antibodies detected in Midwestern white-tailed deer (Odocoileus virginianus) by Western blot and ELISA [J].

Anderson, Todd ;

DeJardin, Amanda ;

Howe, Daniel K. ;

Dubey, J. P. ;

Michalski, Michelle L. .

VETERINARY PARASITOLOGY, 2007, 145 (1-2) :152-155

[3] Prevalence of Neospora caninum and Toxoplasma gondii antibodies in wild ruminants from the countryside or captivity in the Czech Republic [J].

Bartova, E. ;

Sedlak, K. ;

Pavlik, I. ;

Literak, I. .

JOURNAL OF PARASITOLOGY, 2007, 93 (05) :1216-1218

[4] IDENTIFICATION AND CHARACTERIZATION OF NEOSPORA-CANINUM TACHYZOITE ANTIGENS USEFUL FOR DIAGNOSIS OF NEOSPOROSIS [J].

BJERKAS, I ;

JENKINS, MC ;

DUBEY, JP .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1994, 1 (02) :214-221

[5] NEOSPORA-CANINUM IN DOGS - DETECTION OF ANTIBODIES BY ELISA USING AN ISCOM ANTIGEN [J].

BJORKMAN, C ;

LUNDEN, A ;

HOLMDAHL, J ;

BARBER, J ;

TREES, AJ ;

UGGLA, A .

PARASITE IMMUNOLOGY, 1994, 16 (12) :643-648

[6] Seroprevalence of Neospora caninum in gray wolves in Scandinavia [J].

Bjorkman, C. ;

Jakubek, E. -B. ;

Arnemo, J. M. ;

Malmsten, J. .

VETERINARY PARASITOLOGY, 2010, 173 (1-2) :139-142

[7]

Cabaj W, 2000, ACTA PARASITOL, V45, P113

[8] Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland [J].

Cabaj, W ;

Moskwa, B ;

Pastusiak, K ;

Gill, J .

VETERINARY PARASITOLOGY, 2005, 128 (1-2) :163-168

[9]

Dubey John P., 2003, Korean Journal of Parasitology, V41, P1

[10] Prevalence of Neospora caninum antibodies in wild ruminants from the Italian Alps [J].

Ferroglio, E ;

Rossi, L .

VETERINARY RECORD, 2001, 148 (24) :754-755

← 1 2 3 →