Eco-friendly synthesis of zinc oxide nanoparticles using Rivina humilis leaf extract and their biomedical applications

被引:19
作者
Annapoorani, Angusamy [1 ]
Koodalingam, Arunagirinadhan [2 ]
Beulaja, Manikandan [3 ]
Saiprasad, Gowrikumar [4 ]
Chitra, Palanivel [5 ]
Stephen, Arumainathan [6 ]
Palanisamy, Subramanian [7 ,8 ]
Prabhu, Narayanasamy Marimuthu [9 ]
You, SangGuan [7 ,8 ]
Janarthanan, Sundaram [1 ]
Manikandan, Ramar [1 ]
机构
[1] Univ Madras, Dept Zool, Guindy Campus, Chennai 600025, Tamil Nadu, India
[2] Sri Theagaraya Coll, PG & Res Dept Zool, Chennai 600021, Tamil Nadu, India
[3] Annai Veilankannis Coll Women, Dept Biochem, Chennai 600015, Tamil Nadu, India
[4] Univ Nebraska Med Ctr, Dept Biochem & Mol Biol, Omaha, NE USA
[5] Univ Nebraska Med Ctr, Dept Internal Med, Div Gastroenterol & Hepatol, Omaha, NE USA
[6] Univ Madras, Dept Nucl Phys, Guindy Campus, Chennai 600025, Tamil Nadu, India
[7] Gangneung Wonju Natl Univ, East Coast Life Sci Inst, 120 Gangneung, Gangwon 25457, South Korea
[8] Gangneung Wonju Natl Univ, Dept Marine Food Sci & Technol, 120 Gangneungdaehangno, Kangnung 25457, Gangwon, South Korea
[9] Alagappa Univ, Dept Anim Hlth & Management, Dis Control & Prevent Lab, Sci Campus, Karaikkudi 630003, Tamil Nadu, India
关键词
Korea; Biosynthesis; Antioxidant; Antibacterial; Neuroblastoma; Anticancer; SILVER NANOPARTICLES; ZNO NANOPARTICLES; GREEN SYNTHESIS; MEDIATED SYNTHESIS; ANTIBACTERIAL ACTIVITY; AZADIRACHTA-INDICA; IN-VITRO; ANTIOXIDANT; ANTICANCER; APOPTOSIS;
D O I
10.1016/j.procbio.2021.11.022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, we synthesized zinc oxide nanoparticles (ZnONPs) using aqueous leaf extract from Rivina humilis and its functions such as antioxidant, antibacterial and anticancer activities were investigated. ZnONPs were further characterized by UV-vis spectrophotometric analysis (UV-vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Field emission scanning electron microscope (FESEM), Transmission electron microscope and Energy dispersive X-ray spectroscopy (EDX). The synthesized ZnONPs exhibited a strong SPR peak at 370 nm. The XRD results confirming that the ZnONPs were crystalline in nature. Electron microscopic data depicted that these nanoparticles were irregular and circular in appearance with a size of 14.4 nm. The ZnONPs exhibited remarkable antioxidant activities than the extract of R. humilis. The antibacterial effect of ZnONPs against bacterial species was determined by agar diffusion assay. Further, Neuro 2a neuroblastoma cells indicated a dose-dependent decrease of viable cells when exposed to ZnONPs (5-100 mu g/ml). ZnONPs induces a huge amount of lipid peroxidation end product malondialdehyde thus driving the cancer cells to be apoptosis. Moreover, treatment of Neuro 2a cells with ZnONPs brings about activation of apoptotic cell death through the intrinsic mitochondrial pathway by overexpressing caspases 3 and 9 while the reduction in Bcl-2 and upregulation of Bax protein.
引用
收藏
页码:192 / 202
页数:11
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