Orpheovirus IHUMI-LCC2: A New Virus among the Giant Viruses

被引：69
作者：
Andreani, Julien [1 ]
Khalil, Jacques Y. B. [1 ,2 ]
Baptiste, Emeline [1 ]
Hasni, Issam [1 ]
Michelle, Caroline [1 ]
Raoult, Didier [1 ]
Levasseur, Anthony [1 ]
La Scola, Bernard [1 ]
机构：
[1] Aix Marseille Univ, IRD, APHM, MEPHI,IHU Mediterranee Infect, Marseille, France
[2] CNRS, Marseille, France
来源：
FRONTIERS IN MICROBIOLOGY | 2018年 / 8卷
关键词：
Orpheovirus; Cedratvirus; Pithovirus; Vermamoeba vermiformis; giant viruses; NCLDV; Orpheoviridae; Pithoviridae; DNA VIRUSES; MIMIVIRUS; GENES; EVOLUTION; AMEBAS; VIROPHAGE; SEQUENCE; GENOMES; ORIGIN; DOMAIN;
D O I：
10.3389/fmicb.2017.02643
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Giant viruses continue to invade the world of virology, in gigantic genome sizes and various particles shapes. Strains discoveries and metagenomic studies make it possible to reveal the complexity of these microorganisms, their origins, ecosystems and putative roles. We isolated from a rat stool sample a new giant virus "Orpheovirus IHUMI-LCC2," using Vermamoeba vermiformis as host cell. In this paper, we describe the main genomic features and replicative cycle of Orpheovirus IHUMI-LCC2. It possesses a circular genome exceeding 1.4 Megabases with 25% G+C content and ovoidal-shaped particles ranging from 900 to 1300 nm. Particles are closed by at least one thick membrane in a single ostiole-like shape in their apex. Phylogenetic analysis and the reciprocal best hit for Orpheovirus show a connection to the proposed Pithoviridae family. However, some genomic characteristics bear witness to a completely divergent evolution for Orpheovirus IHUMI-LCC2 when compared to Cedratviruses or Pithoviruses.
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页数：11
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共 57 条
[1]   The rapidly expanding universe of giant viruses: Mimivirus, Pandoravirus, Pithovirus and Mollivirus [J].
Abergel, Chantal ;
Legendre, Matthieu ;
Claverie, Jean-Michel .
FEMS MICROBIOLOGY REVIEWS, 2015, 39 (06) :779-796
[2]   The analysis of translation-related gene set boosts debates around origin and evolution of mimiviruses [J].
Abrahao, Jonatas Santos ;
Araujo, Rodrigo ;
Colson, Philippe ;
La Scola, Bernard .
PLOS GENETICS, 2017, 13 (02)
[3]   Giant Viruses of Amoebas: An Update [J].
Aherfi, Sarah ;
Colson, Philippe ;
La Scola, Bernard ;
Raoult, Didier .
FRONTIERS IN MICROBIOLOGY, 2016, 7
[4]   Pacmanvirus, a New Giant Icosahedral Virus at the Crossroads between Asfarviridae and Faustoviruses [J].
Andreani, Julien ;
Khalil, Jacques Yaacoub Bou ;
Sevvana, Madhumati ;
Benamar, Samia ;
Di Pinto, Fabrizio ;
Bitam, Idir ;
Colson, Philippe ;
Klose, Thomas ;
Rossmann, Michael G. ;
Raoult, Didier ;
La Scola, Bernard .
JOURNAL OF VIROLOGY, 2017, 91 (14)
[5]   Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses [J].
Andreani, Julien ;
Aherfi, Sarah ;
Khalil, Jacques Yaacoub Bou ;
Di Pinto, Fabrizio ;
Bitam, Idir ;
Raoult, Didier ;
Colson, Philippe ;
La Scola, Bernard .
VIRUSES-BASEL, 2016, 8 (11)
[6]   Whole-Genome Sequencing of a Pandoravirus Isolated from Keratitis-Inducing Acanthamoeba [J].
Antwerpen, M. H. ;
Georgi, E. ;
Zoeller, L. ;
Woelfel, R. ;
Stoecker, K. ;
Scheid, P. .
GENOME ANNOUNCEMENTS, 2015, 3 (02)
[7]   Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae [J].
Arslan, Defne ;
Legendre, Matthieu ;
Seltzer, Virginie ;
Abergel, Chantal ;
Claverie, Jean-Michel .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (42) :17486-17491
[8]   Faustoviruses: Comparative Genomics of New Megavirales Family Members [J].
Benamar, Samia ;
Reteno, Dorine G. I. ;
Bandaly, Victor ;
Labas, Noemie ;
Raoult, Didier ;
La Scola, Bernard .
FRONTIERS IN MICROBIOLOGY, 2016, 7
[9]   Cedratvirus lausannensis - digging into Pithoviridae diversity [J].
Bertelli, Claire ;
Mueller, Linda ;
Thomas, Vincent ;
Pillonel, Trestan ;
Jacquier, Nicolas ;
Greub, Gilbert .
ENVIRONMENTAL MICROBIOLOGY, 2017, 19 (10) :4022-4034
[10]   GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions [J].
Besemer, J ;
Lomsadze, A ;
Borodovsky, M .
NUCLEIC ACIDS RESEARCH, 2001, 29 (12) :2607-2618
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