CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis

被引:7
作者
Yang, Jian [1 ]
Tian, Shuaiwei [1 ]
Wang, Baocheng [1 ]
Wang, Jiajia [1 ]
Cao, Liangliang [1 ]
Wang, Qinhua [1 ]
Xie, Wanqun [1 ]
Liang, Zhuangzhuang [1 ]
Zhao, Heng [1 ]
Zhao, Yang [1 ]
Liao, Keman [2 ,3 ]
Ma, Jie [1 ]
机构
[1] Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Dept Pediat Neurosurg, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Brain Injury Ctr, Shanghai, Peoples R China
[3] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Neurosurg, Shanghai, Peoples R China
基金
美国国家科学基金会;
关键词
glioblastoma; miR-877-5p; FOXM1; competing endogenous RNA (CeRNA); CELL LUNG-CANCER; MESENCHYMAL TRANSITION; PROMOTES TUMORIGENESIS; FOXM1; EXPRESSION; METASTASIS; INVASION; GROWTH; LOOP;
D O I
10.3389/fonc.2021.801776
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Glioblastoma is a rare yet lethal type of tumor that poses a crucible for the medical profession, owing to its rapid proliferation and invasion resulting in poor prognosis. Circular RNAs (circRNAs), a subclass of regulatory RNAs, are implicated in the regulation of cancerous progression. This study aims to investigate the roles and underlying mechanism of circPIK3C2A in regulating proliferation and invasion of glioblastoma. qRT-PCR assays showed that the expression level of circPIK3C2A was aberrantly higher in glioblastoma cell lines, in comparison with that in normal glia cells. The ectopic expression of circPIK3C2A promoted the proliferation, invasion and clonal formation of glioblastoma cells, while circPIK3C2A loss-of-function exerted exactly the opposite biological effects on the cells. The construction of subcutaneous xenograft tumor model in nude mice indicated that circPIK3C2A loss-of-function effectively diminished tumor load in vivo and prolonged the survival time of tumor-bearing animals. Luciferase reporter assay confirmed the interaction among circPIK3C2A/miR-877-5p and FOXM1. CircPIK3C2A function as competitive endogenous RNA via sponging miR-877-5p through certain binding sites, thereby modulating the expression of FOXM1. Our results collectively indicate that circPIK3C2A functions as ceRNA by mediating miR-877-5p/FOXM1 axis, providing a novel perspective of applying CircPIK3C2A in the clinical intervention of glioblastoma in the future.
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页数:13
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