Bovine lactoferrin allergenicity as studied in murine model of allergy

In our study, we tried to investigate the allergenic properties of bovine lactoferrin (bLf) at different doses. The lactoferrin (LF) allergenicity was explored using murine model of allergy through measuring anti-LF IgG, IgG1 and IgE antibodies (Abs) responses and by in vivo anaphylactic reactions in LF sensitized mice. A histological examination of intestinal lamina propria was performed to assess gut inflammation. Four groups of mice (Balb/c) were sensitized intraperitoneally to bLf using different doses (1%, 2%, 5% and 10%). Histological study of intestinal lamina propria revealed a marked decrease in villus length in 5% and 10% sensitized mice groups. Also, high levels of anti-bLf IgG and IgE were observed. Concerning the in vivo reactions, all groups developed clinical symptoms of anaphylactic reactions at different stages. Our findings show that LF-sensitized mice at 5% and 10% developed important clinical symptoms after intraperitoneal challenge with LF.