Effects of polymorphisms in endothelial nitric oxide synthase and folate metabolizing genes on the concentration of serum nitrate, folate, and plasma total homocysteine after folic acid supplementation: A double-blind crossover study

被引：10
作者：
Cabo, Rona [1 ]
Hernes, Sigrunn [2 ]
Slettan, Audun [1 ]
Haugen, Margaretha [2 ]
Ye, Shu [3 ]
Blomhoff, Rune [4 ,5 ]
Mansoor, M. Azam [1 ]
机构：
[1] Univ Agder, Dept Nat Sci, Kristiansand, Norway
[2] Univ Agder, Dept Publ Hlth Sport & Nutr, Kristiansand, Norway
[3] Queen Mary Univ London, William Harvey Res Inst, London, England
[4] Univ Oslo, Dept Nutr, Oslo, Norway
[5] Oslo Univ Hosp, Dept Clin Serv, Clin Canc Med Transplantat & Surg, Oslo, Norway
来源：
NUTRITION | 2015年 / 31卷 / 02期
关键词：
Genetic polymorphisms; HPLC; Serum nitrate; Serum folate; Plasma total homocysteine; DIHYDROFOLATE-REDUCTASE DHFR; CORONARY-ARTERY-DISEASE; DIETARY NITRATE; DELETION POLYMORPHISM; RISK-FACTOR; DIHYDROPTERIN REDUCTASE; BLOOD-PRESSURE; SPINA-BIFIDA; IN-VIVO; SMOKING;
D O I：
10.1016/j.nut.2014.08.009
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
Objectives: A number of studies have explored the effects of dietary nitrate on human health. Nitrate in the blood can be recycled to nitric oxide, which is an essential mediator involved in many important biochemical mechanisms. Nitric oxide is also formed in the body from L-arginine by nitric oxide synthase. The aim of this study was to investigate whether genetic polymorphisms in endothelial nitric oxide synthase (eNOS) and genes involved in folate metabolism affect the concentration of serum nitrate, serum folate, and plasma total homocysteine in healthy individuals after folic acid supplementation. Methods: In a randomized double-blind, crossover study, participants were given either folic acid 800 mu g/d (n = 52) or placebo (n = 51) for 2 wk. Wash-out period was 2 wk. Fasting blood samples were collected, DNA was extracted by salting-out method and the polymorphisms in eNOS synthase and folate genes were genotyped by polymerase chain reaction methods. Measurement of serum nitrate and plasma total homocysteine (p-tHcy) concentration was done by high-performance liquid chromatography. Results: The concentration of serum nitrate did not change in individuals after folic acid supplements (trial 1); however, the concentration of serum nitrate increased in the same individuals after placebo (P = 0.01) (trial 2). The individuals with three polymorphisms in eNOS gene had increased concentration of serum folate and decreased concentration of p-tHcy after folic acid supplementation. Among the seven polymorphisms tested in folate metabolizing genes, serum nitrate concentration was significantly decreased only in DHFR del 19 gene variant. A significant difference in the concentration of serum nitrate was detected among individuals with MTHFR C > T677 polymorphisms. Conclusions: Polymorphisms in eNOS and folate genes affect the concentration of serum folate and p-tHcy but do not have any effect on the concentration of NO3 in healthy individuals after folic acid supplementation. (C) 2014 Elsevier Inc. All rights reserved. 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：337 / 344
页数：8
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