Agrobacterium mediated transformation of Tunisian Cucumis melo cv. maazoun

被引：11
作者：
Rhimi, Awatef
Hernould, Michel
Boussaid, Mohamed
机构：
[1] INSAT, Lab Plant Biotechnol, Dept Biol, Ctr Urbain Nord, Tunis, Tunisia
[2] INRA, IBVM, UMR CRA Bordeaux 71 619, Lab Cellular Biol & Plant Biotechnol, Villenave Dornon, France
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2007年 / 6卷 / 18期
关键词：
Cucumis melo; genetic transformation; kanamycin; culture medium; PCR analysis;
D O I：
10.5897/AJB2007.000-2338
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Transgenic Cucumis melo cv. Maazoun containing the neomycin phosphotransferase II (NPT II) chimeric gene conferring resistance to kanamycin were obtained from cotyledons explants inoculated with Agrobacterium tumefaciens (GV3101) that contained the binary vector plasmid pADI. Transformed shoots were obtained on Murashige and Skoog medium supplemented with 1.50 mg l(-1) IAA, 0.10 mg l(-1) BAP, 0.01 mg l(-1) NAA and 6 mg l(-1) kinetin. Transformants were selected by using only 100 mg l(-1) of kanamycin and 4 days of pre-culture. Putative transformants were confirmed for transgene insertion through polymerase chain reaction (PCR) analysis. From the inoculated explants, 6.66% produced transgenic shoots.
引用
收藏
页码：2162 / 2165
页数：4
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