Alginate beads encapsulation matrix for urease and polyethyleneglycol-urease

被引：10

作者：

Baysal, Senay Hamarat ^{[1
]}

机构：

[1] Ege Univ, Fen Fak, TR-35100 Bornova, Turkey

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2007年 / 35卷 / 04期

关键词：

alginate; polyethyleneglycol-5000; urea removal; urease;

D O I：

10.1080/10731190701460374

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Urease was immobilized activated PEG-5000 and encapsulated urease and PEG modified urease within alginate beads. Encapsulated urease and PEG-urease were thoroughly characterized for pH, temperature and stabilities and these properties were compared with free and PEG modified enzyme. A 34 and 57% mass and 19.3 and 43% activity yield of urease and PEG-urease resulted following encapsulation. The average diameter for the beads was found to be 2.5 mm for urease bead and 1.8 mm for PEG-urease bead. The stabilities of PEG-urease beads at pH 6.0 were higher than those of urease beads. PEG-urease beads retained 70% of initial activity after reusing seven times at pH 6.0. Also time dependency of substrate conversion was determined for enzyme beads.

引用

页码：457 / 465

页数：9

共 14 条

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