Alginate beads encapsulation matrix for urease and polyethyleneglycol-urease

被引：10
作者：
Baysal, Senay Hamarat [1 ]
机构：
[1] Ege Univ, Fen Fak, TR-35100 Bornova, Turkey
来源：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2007年 / 35卷 / 04期
关键词：
alginate; polyethyleneglycol-5000; urea removal; urease;
D O I：
10.1080/10731190701460374
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Urease was immobilized activated PEG-5000 and encapsulated urease and PEG modified urease within alginate beads. Encapsulated urease and PEG-urease were thoroughly characterized for pH, temperature and stabilities and these properties were compared with free and PEG modified enzyme. A 34 and 57% mass and 19.3 and 43% activity yield of urease and PEG-urease resulted following encapsulation. The average diameter for the beads was found to be 2.5 mm for urease bead and 1.8 mm for PEG-urease bead. The stabilities of PEG-urease beads at pH 6.0 were higher than those of urease beads. PEG-urease beads retained 70% of initial activity after reusing seven times at pH 6.0. Also time dependency of substrate conversion was determined for enzyme beads.
引用
收藏
页码：457 / 465
页数：9
相关论文
共 14 条
[1]   Preparation and application of spacer-arm-attached poly (hydroxyethyl methacrylate-co-glycidyl methacrylate) films for urease immobilisation [J].
Bayramoglu, G ;
Altinok, H ;
Bulut, A ;
Denizli, A ;
Arica, MY .
REACTIVE & FUNCTIONAL POLYMERS, 2003, 56 (02) :111-121
[2]   In vitro study of urease/AlaDH enzyme system encapsulated into human erythrocytes and research into its medical applications [J].
Baysal, SH ;
Uslan, AH .
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, 2002, 30 (01) :71-77
[3]   Immobilization of urease from pigeonpea (Cajanus cajan L.) in polyacrylamide gels and calcium alginate beads [J].
Das, N ;
Kayastha, AM ;
Malhotra, OP .
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1998, 27 :25-29
[4]   Protein loss by the microencapsulation of an enzyme (lactase) in alginate beads [J].
Dashevsky, A .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1998, 161 (01) :1-5
[5]  
ELCIN M, 1992, BIOORGAN MED CHEM, V5, P31
[6]   MANUAL AND AUTOMATED METHODS FOR MEASURING UREA BASED ON A MODIFICATION OF ITS REACTION WITH DIACETYL MONOXIME AND THIOSEMICARBAZIDE [J].
EVANS, RT .
JOURNAL OF CLINICAL PATHOLOGY, 1968, 21 (04) :527-&
[7]   IMMOBILIZATION OF POLY(ETHYLENE OXIDE) ON POLY(ETHYLENE-TEREPHTHALATE) USING A PLASMA POLYMERIZATION PROCESS [J].
GOMBOTZ, WR ;
GUANGHUI, W ;
HOFFMAN, AS .
JOURNAL OF APPLIED POLYMER SCIENCE, 1989, 37 (01) :91-107
[8]   Immobilization of urease on activated methoxypolyethyleneglycol-5000 [J].
Hamarat, S ;
Uslan, AH .
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, 1996, 24 (03) :273-283
[9]   Poly(methylene co-guanidine) coated alginate as an encapsulation matrix for urease [J].
Hearn, E ;
Neufeld, RJ .
PROCESS BIOCHEMISTRY, 2000, 35 (10) :1253-1260
[10]  
IMREN H, 1985, KLIN TANIDA LAB, V637, P716
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