VALIDATION AND UNCERTAINTY ESTIMATION OF A METHOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE ANALYSIS OF LYCOPENE AND β-CAROTENE IN TOMATO PULP

被引：5

作者：

Alves, Adriana Barreto ^{[1
]}

da Silva, Marta Gomes ^{[1
]}

Nogueira Carvalho, Paulo Roberto ^{[1
]}

Vissotto, Lizziane Cynara ^{[1
]}

Bragagnolo, Neura ^{[2
]}

机构：

[1] Inst Tecnol Alimentos, Ctr Ciencia & Qualidade Alimentos, BR-13070178 Campinas, SP, Brazil

[2] Univ Estadual Campinas, Fac Engn Alimentos, Dept Ciencia Alimentos, BR-13083862 Campinas, SP, Brazil

来源：

QUIMICA NOVA | 2010年 / 33卷 / 09期

关键词：

tomato pulp; validation; uncertainty estimation; HPLC METHOD; FOODS;

D O I：

10.1590/S0100-40422010000900025

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

VALIDATION AND UNCERTAINTY ESTIMATION OF A METHOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE ANALYSIS OF LYCOPENE AND beta-CAROTENE IN TOMATO PULP. A method to quantify lycopene and beta-carotene in freeze dried tomato pulp by high performance liquid chromatography (HLPC) was validated according to the criteria of selectivity, sensitivity, precision and accuracy. and uncertainty estimation of measurement was determined with data obtained in the validation. The validated method presented is selective in terms of analysis, and it had a good precision and accuracy. Detection limit for lycopene and beta-carotene was 4.2 and 0.23 mg 100 g(-1), respectively. The estimation of expanded uncertainty (K = 2) for lycopene was 104 +/- 21 mg 100 g(-1) and for beta-carotene was 6.4 +/- 1.5 mg 100 g(-1).

引用

页码：1962 / 1966

页数：5

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