Isolation, purification and physicochemical characterization of water-soluble Bacillus thuringiensis melanin

被引：82

作者：

Aghajanyan, AE ^{[1
]}

Hambardzumyan, AA ^{[1
]}

Hovsepyan, AS ^{[1
]}

Asaturian, RA ^{[1
]}

Vardanyan, AA ^{[1
]}

Saghiyan, AA ^{[1
]}

机构：

[1] Inst Biotechnol, JSC, Yerevan 375056, Armenia

来源：

PIGMENT CELL RESEARCH | 2005年 / 18卷 / 02期

关键词：

melanin; Bacillus thuringiensis; purification; infrared spectra; electron paramagnetic resonance spectra; molecular weight; isoelectric point;

D O I：

10.1111/j.1600-0749.2005.00211.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Melanins are widely used in medicine, pharmacology, cosmetics and other fields. Although several technologies for the purification of water-insoluble dioxyphenylalanine (DOPA) melanins have been described, a source of water-soluble melanin is highly desirable. Here we describe an effective procedure for the isolation and purification of water-soluble melanin using the culture medium of Bacillus thuringiensis subsp. galleriae strain K1. Water-soluble melanin from this organism has an isoelectric point (pI = 3.0-3.2) and was purified optimally by adsorbtion using the IA-1r resin and elution as a concentrated solution. The purified melanin obtained exhibited a similar infra-red absorbtion spectrum to synthetic melanin and contained quinolic and phenolic structures and an amino acid content of around 20% after acid hydrolysis. The molecular weight of the purified melanin determined by SDS-PAGE was 4 kDa and the electromagnetic spin resonance spectrum of the purified microbial melanin was a slightly asymmetric singlet without hyperfine structure with about 7 Gauss width of the line between points of the maximum incline and g = 2.006. The concentration of paramagnetic centers in melanin is 0.21 x 10(18) spin/g. The results obtained provide a rapid, simple and inexpensive method for the large scale purification of water soluble melanin that may have widespread applications.

引用

页码：130 / 135

页数：6

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