Prolyl endopeptidase inhibitory activity of chitosan sulfates with different degree of deacetylation

被引：23
作者：
Je, JY
Park, PJ
Kim, SK
机构：
[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea
[2] Konkuk Univ, Dept Biotechnol, Chungju 380701, South Korea
来源：
CARBOHYDRATE POLYMERS | 2005年 / 60卷 / 04期
关键词：
hetero-chitosans; sulfated derivatives; prolyl endopeptidase; competitive;
D O I：
10.1016/j.carbpol.2005.03.007
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Three kinds of partially deacetylated chitosan, 90% deacetylated chitosan, 75% deacetylated chitosan and 50% deacetylated chitosan, were prepared from crab chitin by N-deacetylation with 40% (w/w) sodium hydroxide solution for different durations. In order to improve biological activity and solubility, their sulfated derivatives were prepared, and prolyl endopeptidase (PEP) inhibitory activities were investigated. Fifty percent-deacetylated chitosan sulfate (50-CS) exhibited the highest inhibitory activity, and inhibition rate was a dose-dependant. In addition, Dixon plots suggested that 50-CS was act as competitive inhibitor, and the inhibition constant (K-i) was 2.6 mg/ml. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：553 / 556
页数：4
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